Koch T R, Roddy D R, Carney J A, Telander R L, Go V L
Gastroenterology Research Unit, Mayo Clinic, Rochester, MN 55905.
Regul Pept. 1988 Jun;21(3-4):309-19. doi: 10.1016/0167-0115(88)90014-6.
A radioimmunoassay for measurement of immunoreactive neuropeptide Y has been developed using antiserum from a rabbit (221) immunized with porcine neuropeptide Y. Antibody 221 has been characterized for both sensitivity and specificity. To determine the distribution of neuropeptide Y in the human gastrointestinal tract, fresh tissue specimens were separated by microdissection into the muscularis externa and the mucosa-submucosa. To examine the origin of neuropeptide Y in human colon, specimens of aganglionic and ganglionic colon were obtained from patients with Hirschsprung's disease. Immunoreactive neuropeptide Y in human gut was present in highest concentrations in the muscularis externa of the stomach and in lowest concentrations in the muscularis externa of the ileum and descending colon. Neuropeptide Y in the stomach was present in higher concentrations in the muscularis externa than in the mucosa-submucosa, but in the descending colon there were lower concentrations of neuropeptide Y in the muscularis externa than in the mucosa-submucosa. In Hirschsprung's disease, concentrations of neuropeptide Y were increased in aganglionic colon in both the muscularis externa and the mucosa-submucosa, compared to corresponding layers from proximal ganglionic colon. Extracts of the gastric muscularis externa and the colonic mucosa-submucosa were separated by C18 reverse-phase high-performance liquid chromatography. One major immunoreactive species was identified by radioimmunoassay which eluted in a position similar to synthetic human neuropeptide Y. These results demonstrated both regional and layer differences in concentrations of neuropeptide Y in human gut. Increased concentrations of neuropeptide Y in aganglionic colon from Hirschsprung's disease most likely result from enlargement of neuropeptide Y-containing extrinsic nerve fibers in both the mucosa-submucosa and the muscularis externa.
已利用用猪神经肽Y免疫的兔（221）抗血清开发出一种用于测量免疫反应性神经肽Y的放射免疫测定法。已对抗体221的敏感性和特异性进行了表征。为了确定神经肽Y在人胃肠道中的分布，通过显微切割将新鲜组织标本分离为肌层和黏膜-黏膜下层。为了研究人结肠中神经肽Y的来源，从患有先天性巨结肠病的患者获取无神经节和有神经节结肠的标本。人肠道中的免疫反应性神经肽Y在胃肌层中的浓度最高，在回肠和降结肠肌层中的浓度最低。胃中的神经肽Y在肌层中的浓度高于黏膜-黏膜下层，但在降结肠中，神经肽Y在肌层中的浓度低于黏膜-黏膜下层。在先天性巨结肠病中，与来自近端有神经节结肠的相应层相比，无神经节结肠的肌层和黏膜-黏膜下层中神经肽Y的浓度均升高。胃肌层和结肠黏膜-黏膜下层的提取物通过C18反相高效液相色谱法进行分离。通过放射免疫测定法鉴定出一种主要的免疫反应性物质，其洗脱位置与合成人神经肽Y相似。这些结果表明人肠道中神经肽Y浓度存在区域和层间差异。先天性巨结肠病无神经节结肠中神经肽Y浓度升高很可能是由于黏膜-黏膜下层和肌层中含神经肽Y的外在神经纤维增多所致。
