Spampinato S, Ferri G L, Candeletti S, Romualdi P, Cavicchini E, Soimero L, Labò G, Ferri S
Institute of Pharmacology, University of Bologna, Italy.
Neuropeptides. 1988 Apr;11(3):101-5. doi: 10.1016/0143-4179(88)90077-7.
Immunoreactive dynorphin A (ir-Dyn A) was detected throughout the human gastrointestinal tract by a validated radioimmunoassay. Moreover, the stability of 125I-Dyn A during extraction procedures was confirmed by high performance liquid chromatography. Levels of ir-Dyn A were higher in the stomach and in the small bowel. In tissue samples separated into the main layers composing the gut wall (muscularis externa, submucosa and mucosa) ir-Dyn A was uniformly distributed. An exception was the colon, where concentrations were higher in the muscular portion. Gel permeation chromatography on samples of mucosa and muscularis externa extracts of ileum and gastric fundus, showed immunoreactive material eluting in several forms of apparently higher molecular weight than Dyn A, while only a minor peak was found to coelute with authentic Dyn A.
通过经过验证的放射免疫分析法在整个人胃肠道中检测到免疫反应性强啡肽A（ir-Dyn A）。此外，通过高效液相色谱法证实了125I-Dyn A在提取过程中的稳定性。胃和小肠中的ir-Dyn A水平较高。在分离成构成肠壁的主要层（外肌层、黏膜下层和黏膜）的组织样本中，ir-Dyn A分布均匀。结肠是个例外，其肌肉部分的浓度较高。对回肠和胃底黏膜及肌层提取物样本进行凝胶渗透色谱分析，结果显示免疫反应性物质以几种明显高于Dyn A分子量的形式洗脱，而仅发现一个小峰与纯Dyn A共洗脱。
