Instituto Federal de Educação, Ciência e Tecnologia de Goiás - IFG, Aparecida de Goiânia, GO, Brasil.
Universidade Federal de Goiás - UFG, Instituto de Patologia Tropical e Saúde Pública - IPTSP, Goiânia, GO, Brasil.
Braz J Biol. 2021 Jun 11;82:e245368. doi: 10.1590/1519-6984.245368. eCollection 2021.
Foliar vegetables contaminated with fecal residues are an important route of transmission of intestinal parasites to humans. The aim of this study was to evaluate the presence of parasitic forms of protozoa and helminths on lettuces (Lactuca sativa) and collard greens (Brassica oleracea) sold in street- and supermarkets in the city of Aparecida de Goiânia, Goiás, Brazil. A total of 30 samples of each vegetable (15 samples from each supermarkets and street markets) was analyzed. All samples were processed by spontaneous sedimentation method and centrifugal flotation. In 45% of the samples, immature forms of intestinal parasites were identified with 66.7% helminths eggs and 33.3% protozoan cysts or oocysts. Significantly more lettuce samples were contaminated with eggs, cysts or oocyst of at least one parasite than collard green samples (U=216; Z=-3.45; P <0.001). The parasitic forms were identified morphologically up to the family level with eggs of Ancylostomatidae, Strongyloididae, Ascarididae and Taeniidae, or oocysts of Eimeriidae, to the genus with Cystoisospora sp. and Toxocara sp., and to the species level with Cystoisospora canis, Dipylidium caninum and Hymenolepis nana. The presence of these infective agents in lettuce and collard green from both street- and supermarkets highlights the high risk of spreading parasites by eating raw vegetables sold in Aparecida de Goiânia.
受粪便污染的茎叶类蔬菜是人类感染肠道寄生虫的重要途径。本研究旨在评估巴西戈亚斯州阿帕雷西达-戈亚尼亚市街头和超市销售的生菜（生菜）和羽衣甘蓝（羽衣甘蓝）上是否存在原生动物和蠕虫寄生虫。分析了每种蔬菜（每个超市和街头市场各 15 个样本）共 30 个样本。所有样本均采用自然沉淀法和离心浮洗法处理。在 45%的样本中，鉴定出未成熟的肠道寄生虫，其中 66.7%为蠕虫卵，33.3%为原生动物囊肿或卵囊。与羽衣甘蓝样本相比，生菜样本中至少有一种寄生虫的卵、囊肿或卵囊污染的比例显著更高（U=216；Z=-3.45；P <0.001）。寄生虫形态学鉴定至科水平，包括Ancylostomatidae、Strongyloididae、Ascarididae 和 Taeniidae 的卵，Eimeriidae 的卵囊，到属水平有 Cystoisospora sp.和 Toxocara sp.，到种水平有 Cystoisospora canis、Dipylidium caninum 和 Hymenolepis nana。生菜和羽衣甘蓝中均存在这些感染因子，表明在阿帕雷西达-戈亚尼亚市食用生蔬菜传播寄生虫的风险很高。
