Araki Masanari, Ohtaki Tadatoshi, Kimura Junpei, Hobo Seiji, Taya Kazuyoshi, Tsunoda Nobuo, Taniyama Hiroyuki, Tsumagari Shigehisa, Nambo Yasuo
The Japan Racing Association, Tokyo 320-0856, Japan.
Department of Veterinary Medicine, College of Bioresource Sciences, Nihon University, Fujisawa, Kanagawa 252-0880, Japan.
J Vet Med Sci. 2021 Jul 28;83(7):1167-1172. doi: 10.1292/jvms.20-0174. Epub 2021 Jun 15.
Immunohistochemical investigations of the expression of surfactant protein A (SP-A) and surfactant protein D (SP-D) in the uterine and placental tissues of 13 pregnant mares were performed using anti-horse monoclonal primary antibodies. Strong positive reactions for both SP-A and SP-D were observed in the trophoblasts in the microcotyledons of the placentae at 182 to 314 days of gestation; in uterine glandular epithelial cells, faint-to-weak reactions were observed during gestation. This study describes, for the first time, the changes in the SP-A and SP-D expression levels in the endometrium of mares during gestation; the SP-A and SP-D expression levels increased after the second trimester of gestation.
使用抗马单克隆一抗对13匹怀孕母马的子宫和胎盘组织中表面活性蛋白A（SP-A）和表面活性蛋白D（SP-D）的表达进行了免疫组织化学研究。在妊娠182至314天的胎盘小叶滋养层细胞中观察到SP-A和SP-D均呈强阳性反应；在子宫腺上皮细胞中，妊娠期间观察到弱阳性至弱反应。本研究首次描述了妊娠期间母马子宫内膜中SP-A和SP-D表达水平的变化；妊娠中期后SP-A和SP-D表达水平升高。
