Department of Head and Neck Surgery, Barretos Cancer Hospital, Barretos, São Paulo, Brazil.
Department of Medicine, Federal University of Rondônia, Rondônia, Brazil.
PLoS One. 2021 Jun 17;16(6):e0253418. doi: 10.1371/journal.pone.0253418. eCollection 2021.
Oropharyngeal cancer is an important public health problem. The aim of our study was to correlatep16 immunohistochemistry in oropharynx squamous cell carcinomas(OPSCC) with clinical and epidemiological features.
We conducted across-sectional study on patients with OPSCC treated at a single institution from 2014 to 2019. Epidemiological and clinical-pathological data were collected from medical records and a questionnaire was applied to determine alcohol consumption, smoking, and sexual behavior. The HPV status was determined by p16 immunohistochemistry.
A total of 252 patients participated in the study, of these 221 (87.7%) were male. There were 81 (32.14%) p16 positive cases and 171 (67.85%) p16 negative cases. The p16positive group was significantly associated with younger patients (50-59 years), higher education level, lower clinical stage and patients who never drank or smoked. Through univariate logistic regression, we observed that female sex (OR, 3.47; 95% CI, 1.60-7.51) and higher education level (OR, 9.39; 95% CI, 2, 81-31,38) were significantly more likely to be p16 positive. Early clinical stage (AJCC8ed) was more associated with p16 positivity both in univariate (OR, 0.14; 95% CI, 0.07-0.26, p<0.001) and multivariate analysis (OR, 0.18; 95% CI, 0.06-0.49, p = 0.001).
This study showed that drinkers and current smokers were less likely to be p16+. Female sex, higher education level and younger age at diagnosis were associated with a higher probability of being p16+. Additionally, there was a higher proportion of patients with early clinical stage (I or II) in the p16 positive group when compared to the p16 negative group.
口咽癌是一个重要的公共卫生问题。我们的研究目的是分析口咽鳞状细胞癌（OPSCC）中 p16 免疫组化与临床和流行病学特征的相关性。
我们对 2014 年至 2019 年在一家机构治疗的 OPSCC 患者进行了一项横断面研究。从病历中收集流行病学和临床病理数据，并应用问卷调查确定酒精摄入、吸烟和性行为。HPV 状态通过 p16 免疫组化确定。
共有 252 例患者参与了这项研究，其中 221 例（87.7%）为男性。81 例（32.14%）p16 阳性，171 例（67.85%）p16 阴性。p16 阳性组与年轻患者（50-59 岁）、受教育程度较高、较低的临床分期和从不饮酒或吸烟的患者显著相关。通过单因素 logistic 回归，我们发现女性（OR，3.47；95%CI，1.60-7.51）和较高的教育程度（OR，9.39；95%CI，2，81-31，38）更有可能呈 p16 阳性。早期临床分期（AJCC8ed）在单因素（OR，0.14；95%CI，0.07-0.26，p<0.001）和多因素分析（OR，0.18；95%CI，0.06-0.49，p = 0.001）中与 p16 阳性更相关。
本研究表明，饮酒者和现吸烟者 p16 阳性的可能性较低。女性、较高的教育程度和较年轻的诊断年龄与更高的 p16 阳性可能性相关。此外，与 p16 阴性组相比，p16 阳性组具有更高比例的早期临床分期（I 期或 II 期）患者。
