Emanuelson Cole, Bardhan Anirban, Deiters Alexander
Department of Chemistry, University of Pittsburgh, Pittsburgh, Pennsylvania 15260, United States.
ACS Synth Biol. 2021 Jul 16;10(7):1682-1689. doi: 10.1021/acssynbio.1c00062. Epub 2021 Jun 18.
DNA-based Boolean logic gates (for example, AND, OR, and NOT) can be assembled into complex computational circuits that generate an output signal in response to specific patterns of oligonucleotide inputs. However, the fundamental nature of NOT gates, which convert the absence of an input into an output, makes their implementation within DNA-based circuits difficult. Premature execution of a NOT gate before completion of its upstream computation introduces an irreversible error into the circuit. By utilizing photocaging groups, we developed a novel DNA gate design that prevents gate function until irradiation at a certain time point. Optical activation provides temporal control over circuit performance by preventing premature computation and is orthogonal to all other components of DNA computation devices. Using this approach, we designed NAND and NOR logic gates that respond to synthetic microRNA sequences. We further demonstrate the utility of the NOT gate within multilayer circuits in response to a specific pattern of four microRNAs.
基于DNA的布尔逻辑门（例如，与门、或门和非门）可以组装成复杂的计算电路，该电路根据特定的寡核苷酸输入模式生成输出信号。然而，非门的基本特性是将输入的缺失转换为输出，这使得在基于DNA的电路中实现它们变得困难。非门在其上游计算完成之前过早执行会给电路引入不可逆的错误。通过利用光笼蔽基团，我们开发了一种新颖的DNA门设计，该设计可防止门功能，直到在特定时间点进行照射。光激活通过防止过早计算来提供对电路性能的时间控制，并且与DNA计算设备的所有其他组件正交。使用这种方法，我们设计了响应合成微小RNA序列的与非门和或非门。我们进一步证明了非门在多层电路中响应四种微小RNA的特定模式时的效用。
