Centre for DNA Nanotechnology (CDNA), Interdisciplinary Nanoscience Center (iNANO), Aarhus University, Aarhus, Denmark; Department of Molecular Biology and Genetics, Aarhus University, Aarhus, Denmark.
Small. 2015 Apr 17;11(15):1811-7. doi: 10.1002/smll.201402755. Epub 2015 Jan 7.
Logic gates are devices that can perform logical operations by transforming a set of inputs into a predictable single detectable output. The hybridization properties, structure, and function of nucleic acids can be used to make DNA-based logic gates. These devices are important modules in molecular computing and biosensing. The ideal logic gate system should provide a wide selection of logical operations, and be integrable in multiple copies into more complex structures. Here we show the successful construction of a small DNA-based logic gate complex that produces fluorescent outputs corresponding to the operation of the six Boolean logic gates AND, NAND, OR, NOR, XOR, and XNOR. The logic gate complex is shown to work also when implemented in a three-dimensional DNA origami box structure, where it controlled the position of the lid in a closed or open position. Implementation of multiple microRNA sensitive DNA locks on one DNA origami box structure enabled fuzzy logical operation that allows biosensing of complex molecular signals. Integrating logic gates with DNA origami systems opens a vast avenue to applications in the fields of nanomedicine for diagnostics and therapeutics.
逻辑门是一种能够通过将一组输入转换为可预测的单个可检测输出来执行逻辑操作的设备。核酸的杂交性质、结构和功能可用于制造基于 DNA 的逻辑门。这些器件是分子计算和生物传感中的重要模块。理想的逻辑门系统应提供广泛的逻辑操作选择，并可集成到多个副本中，以构建更复杂的结构。在这里，我们成功构建了一个小型基于 DNA 的逻辑门复合物，该复合物产生与六个布尔逻辑门（AND、NAND、OR、NOR、XOR 和 XNOR）的操作相对应的荧光输出。该逻辑门复合物在三维 DNA 折纸盒结构中也能正常工作，在该结构中，它控制盖子处于关闭或打开位置。在一个 DNA 折纸盒结构上实现多个 miRNA 敏感 DNA 锁，可实现模糊逻辑操作，从而能够对复杂的分子信号进行生物传感。将逻辑门与 DNA 折纸系统集成在一起，为纳米医学领域的诊断和治疗应用开辟了广阔的途径。
