Han Fang, Zhou Dian-Bing, Song Wei, Hu Yan-Yun, Lv Ya-Ning, Ding Lei, Zheng Ping, Jia Xue-Ying, Zhang Lei, Deng Xiao-Jun
Technology Center of Hefei Customs, and Anhui Province Key Laboratory of Analysis and Detection for Food Safety, Hefei, Anhui 230022, China.
Instruments' Center for Physical Science, University of Science and Technology of China, Hefei, Anhui 230026, China.
J Chromatogr A. 2021 Aug 16;1651:462321. doi: 10.1016/j.chroma.2021.462321. Epub 2021 Jun 3.
A high-efficiency approach for the synthesis of molecularly imprinted polymers has been developed and further for the solid-phase extraction of sulfonylurea herbicides in food samples. Molecular simulation approach combined chemometric selected metsulfuron-methyl (MSM) and 2-trifluoromethyl acrylic acid (TFMAA) as the template and the monomer to synthesize the molecularly imprinted polymers (MIPs). Experimental validation confirmed that the MSM-imprinted polymers showed a higher selectivity and affinity to sulfonylurea herbicides. The optimized molecularly imprinted solid-phase extraction (MISPE) conditions, including loading, washing, and eluting conditions, were established. The developed MISPE technology combined HPLC-MSMS was successfully used for the determination of sulfonylurea herbicides in foods. Compared with commercial SPE columns, MISPE showed high affinity, excellent selectivity and low matrix effect. The recoveries of sulfonylurea herbicides spiked in four matrices were between 86.4% and 100.2%, with the relative standard deviations (RSD) in the range of 0.9%-10.5%.
已开发出一种高效合成分子印迹聚合物的方法，并将其进一步用于食品样品中磺酰脲类除草剂的固相萃取。分子模拟方法结合化学计量学选择甲磺隆（MSM）和2-三氟甲基丙烯酸（TFMAA）作为模板和单体来合成分子印迹聚合物（MIPs）。实验验证证实，MSM印迹聚合物对磺酰脲类除草剂表现出更高的选择性和亲和力。建立了优化的分子印迹固相萃取（MISPE）条件，包括上样、洗涤和洗脱条件。所开发的MISPE技术与HPLC-MSMS联用成功用于食品中磺酰脲类除草剂的测定。与商用SPE柱相比，MISPE表现出高亲和力、优异的选择性和低基质效应。在四种基质中加标的磺酰脲类除草剂的回收率在86.4%至100.2%之间，相对标准偏差（RSD）在0.9%至10.5%范围内。
