评价原儿茶醛对肠侵袭性大肠埃希菌的膜损伤机制及在猪肉中的抑菌效果模拟

Evaluation of the membrane damage mechanism of protocatechualdehyde against Yersinia enterocolitica and simulation of growth inhibition in pork.

机构信息

College of Food and Bioengineering, Shaanxi University of Science and Technology, Xi'an, Shaanxi 710021, China.

出版信息

Food Chem. 2021 Nov 30;363:130340. doi: 10.1016/j.foodchem.2021.130340. Epub 2021 Jun 9.

DOI:10.1016/j.foodchem.2021.130340

Abstract

This study sought to explore the antibacterial mechanism associated with membrane damage in Yersinia enterocolitica by protocatechualdehyde (PCA), thus providing improved knowledge of whether PCA is suitable for pork preservation. The minimum inhibitory concentration (MIC) of PCA was determined by micro-broth dilution. We then characterized functional and morphological changes of Y. enterocolitica treated with PCA. Finally, the growth inhibition model of PCA against Y. enterocolitica in pork was established using the response surface method. Accordingly, the MIC of PCA against Y. enterocolitica was found to be 0.3125 mg/mL. Significant observations incorporated membrane depolarization, a markedly decreased intracellular ATP and pH, and morphological changes induced by PCA treatment. After PCA treatment under low temperatures, the average Y. enterocolitica count in pork decreased by two log cycles. According to the obtained findings, PCA exhibited satisfactory performances as a food preservative to control the growth and reproduction of Y. enterocolitica in pork.

摘要

本研究旨在探讨原儿茶醛（PCA）对肠侵袭性大肠埃希菌的膜损伤相关的抗菌机制，从而为 PCA 是否适合猪肉保鲜提供更深入的了解。通过微量肉汤稀释法确定 PCA 的最小抑菌浓度（MIC）。然后，我们对 PCA 处理后的肠侵袭性大肠埃希菌的功能和形态变化进行了表征。最后，采用响应面法建立了 PCA 对猪肉中肠侵袭性大肠埃希菌的生长抑制模型。结果表明，PCA 对肠侵袭性大肠埃希菌的 MIC 为 0.3125mg/mL。我们观察到 PCA 处理后，细胞膜去极化、细胞内 ATP 和 pH 值显著下降以及形态发生变化。在低温下 PCA 处理后，猪肉中肠侵袭性大肠埃希菌的平均数量减少了两个对数周期。根据研究结果，PCA 作为一种食品防腐剂，在控制猪肉中肠侵袭性大肠埃希菌的生长和繁殖方面表现出良好的性能。