Akatov V S, Lavrovskaia V P
Tsitologiia. 1991;33(1):64-72.
Chinese hamster fibroblasts (CHF) and NIH 3T3 cells were cultured on a glass substrate at different distances from the porous membrane separating the cells from the perfusing medium. It is shown that with perfusion of medium above the membrane there is no movement of the medium near the cells. In both the types of culture, the cells grow in multilayers, however the multilayer character of growth in CHF is more pronounced than in NIH 3T3 cells. The saturation density of the cultures depends on the cell-membrane separation, and at separations of no more than 0.2 mm exceeds the saturation density in the monolayer by 8-10 fold. The dependences of the saturation density on separation are different for CHE and NIH 3T3 cells, indicating qualitative differences in the inhibition of cell growth in monolayers between these cultures. The results obtained indicate that the inhibition of cell growth in monolayer is due to mass exchange limitations, rather than to intercellular contact interactions.
将中国仓鼠成纤维细胞（CHF）和NIH 3T3细胞培养在玻璃基质上，使其与灌注培养基的多孔膜保持不同距离。结果表明，当在膜上方灌注培养基时，细胞附近的培养基没有流动。在这两种培养类型中，细胞均呈多层生长，然而CHF中的多层生长特征比NIH 3T3细胞更为明显。培养物的饱和密度取决于细胞与膜的间距，当间距不超过0.2毫米时，其饱和密度比单层培养时高出8至10倍。CHE和NIH 3T3细胞的饱和密度与间距的关系不同，这表明这些培养物在单层细胞生长抑制方面存在质的差异。所得结果表明，单层细胞生长的抑制是由于物质交换限制，而非细胞间接触相互作用。
