College of Food Science and Technology, Nanjing Agricultural University, Nanjing, PR China.
J Sci Food Agric. 2022 Jan 30;102(2):628-643. doi: 10.1002/jsfa.11392. Epub 2021 Jul 10.
Heat shock transcription factors (Hsfs) play pivotal roles in plant responses to stress. Although glycine betaine (GB) and hot water (HW) treatments are effective in reducing chilling injury (CI), little is known about the characterization of the Hsfs gene family and its potential roles in alleviating CI by regulating antioxidant systems in peach fruit.
In this study, 17 PpHsfs were identified in the peach genome and were investigated using bioinformatics, including chromosomal locations, phylogenetic relationships, gene structure, motifs, and promoter analyses. The expression patterns of PpHsfs under GB and HW treatments were also investigated. The PpHsfs showed different expression patterns in GB- and HW-treated fruit, and most of them were significantly up-regulated by both treatments, especially PpHsfA1a/b, PpHsfA2a, PpHsfA9a, and PpHsfB2a/b. Meanwhile, GB and HW treatments induced higher levels of gene expression and antioxidant enzyme activity of superoxide dismutase (SOD), catalase (CAT), and ascorbate peroxidase (APX) compared to the control, contributing to the inhibition of hydrogen peroxide (H O ) accumulation and superoxide anion (O ) production. Moreover, the correlation analysis between PpHsfs and antioxidant-related genes showed that three PpAPXs were significantly correlated with ten PpHsfs, whereas PpCAT and PpSOD had no significant correlations with PpHsfs, which indicated that PpAPX might be regulated by PpHsfs.
The results indicated that GB and HW treatments induced different PpHsfs transcript levels to regulate the antioxidant gene expressions, which might be beneficial in inhibiting the accumulation of reactive oxygen species and protecting the integrity of cell structure, thus alleviating the development of CI in peach fruit during cold storage. © 2021 Society of Chemical Industry.
热休克转录因子（Hsfs）在植物应对胁迫的反应中发挥着关键作用。尽管甘氨酸甜菜碱（GB）和热水（HW）处理可有效降低冷害（CI），但对于 Hsfs 基因家族的特征及其通过调节桃果实抗氧化系统缓解 CI 的潜在作用知之甚少。
本研究在桃基因组中鉴定了 17 个 PpHsfs，通过生物信息学方法对其进行了研究，包括染色体定位、系统发育关系、基因结构、基序和启动子分析。还研究了 PpHsfs 在 GB 和 HW 处理下的表达模式。PpHsfs 在 GB 和 HW 处理的果实中表现出不同的表达模式，其中大多数在两种处理下均显著上调，尤其是 PpHsfA1a/b、PpHsfA2a、PpHsfA9a 和 PpHsfB2a/b。同时，GB 和 HW 处理诱导了超氧化物歧化酶（SOD）、过氧化氢酶（CAT）和抗坏血酸过氧化物酶（APX）的基因表达和抗氧化酶活性更高，与对照相比，抑制了过氧化氢（H2O2）的积累和超氧阴离子（O2-）的产生。此外，PpHsfs 与抗氧化相关基因的相关性分析表明，三个 PpAPXs 与十个 PpHsfs 显著相关，而 PpCAT 和 PpSOD 与 PpHsfs 无显著相关性，这表明 PpAPX 可能受 PpHsfs 调控。
结果表明，GB 和 HW 处理诱导不同的 PpHsfs 转录水平来调节抗氧化基因表达，这可能有助于抑制活性氧的积累，保护细胞结构的完整性，从而缓解桃果实冷藏过程中 CI 的发生。© 2021 化学工业协会。
