Antioxidant Polyphenols Team, Department of Food Engineering, Sichuan University, Chengdu, PR China.
The Key Laboratory of Food Science and Technology of Sichuan Province of Education, Sichuan University, Chengdu, PR China.
J Food Sci. 2021 Jul;86(7):2910-2923. doi: 10.1111/1750-3841.15801. Epub 2021 Jun 19.
The effect of pine needle extract from Cedrus deodara (PNE) on the quality of salted meat was reported, and its action mechanism was further investigated. With the treatment of PNE, the physicochemical properties of salted meat were improved. The peroxide value decreased from 16.18 to 6.78 mmol O /kg, while the thiobarbituric acid value decreased from 0.79 to 0.40 mg MDA/kg. Moreover, the salted meat with PNE also had the better texture, color, and volatile compositions. The 0.2% PNE group showed the highest ΔE value (63.16 ± 0.56), hardness (813.5 ± 48.7 g), and volatility (45.86 ± 0.39), while the control group showed the lowest ΔE value (43.92 ± 2.13), hardness (515.8 ± 17.3 g) and volatility (29.97 ± 0.56). In addition, with the analysis of fluorescence and circular dichroism spectroscopy, the spatial structures of myofibrillar protein (MP) in salted meat were obviously changed by PNE. Meanwhile, methylconiferin, 1-O-feruloyl-β-D-glucose, nortrachelogenin, secoxyloganin, 1-O-(4-coumaroyl)-β-D-glucose and pelargonidin-3-O-glucoside were identified from PNE. Furthermore, according to the analysis of molecular docking, hydrogen bond, hydrophobic force, and electrostatic force were obtained as the main molecular forces between MP and the phenolic compounds of PNE, while arginine, glutamic acid, and glycine residues were the main binding sites. All results suggested that PNE might be a potential candidate to improve the quality of salted meat in the food industry. PRACTICAL APPLICATION: The quality deterioration of meat may not only affect its further processing and consumption but also may lead to some food safety problems. In present study, PNE exhibited the fine capability to inhibit the oxidation of meat, while it could ameliorate the texture, color, and physicochemical properties of meat due to its tightly interaction with myofibrillar protein. All result suggested that PNE could be potentially utilized to improve the quality of meat in food industry.
报道了雪松松针提取物(PNE)对咸肉品质的影响,并进一步研究了其作用机制。用 PNE 处理后,改善了咸肉的理化性质。过氧化物值从 16.18mmol O/kg 降低到 6.78mmol O/kg,而硫代巴比妥酸值从 0.79mg MDA/kg 降低到 0.40mg MDA/kg。此外,用 PNE 处理的咸肉质地、颜色和挥发性成分也更好。0.2%PNE 组的ΔE 值(63.16±0.56)、硬度(813.5±48.7g)和挥发性(45.86±0.39)最高,而对照组的ΔE 值(43.92±2.13)、硬度(515.8±17.3g)和挥发性(29.97±0.56)最低。此外,通过荧光和圆二色光谱分析,PNE 明显改变了腌制肉中肌原纤维蛋白(MP)的空间结构。同时,从 PNE 中鉴定出甲基柏木脂素、1-O-阿魏酰-β-D-葡萄糖、北美鹅掌楸素、secoxyloganin、1-O-(4-香豆酰)-β-D-葡萄糖和pelargonidin-3-O-葡萄糖苷。此外,根据分子对接分析,氢键、疏水作用力和静电力是 MP 与 PNE 中酚类化合物之间的主要分子力,而精氨酸、谷氨酸和甘氨酸残基是主要的结合位点。所有结果表明,PNE 可能是改善食品工业中咸肉品质的潜在候选物。 实际应用:肉的质量恶化不仅会影响其进一步加工和消费,还可能导致一些食品安全问题。在本研究中,PNE 表现出良好的抑制肉氧化能力,同时由于其与肌原纤维蛋白的紧密相互作用,改善了肉的质地、颜色和理化性质。所有结果表明,PNE 可潜在用于改善食品工业中肉的品质。