Dept of Food Science and Technology and author Feng is with College of Life Science, Sichuan Univ, Chengdu 610065, PR China.
J Food Sci. 2011 Mar;76(2):C318-23. doi: 10.1111/j.1750-3841.2010.02023.x.
The antibrowning and antimicrobial activities of the water-soluble extract from pine needles of Cedrus deodara (CDE), a traditional Chinese medicine and raw materials of pine needle tea, was investigated. Total phenols of CDE were 31.4 ± 0.53 mg gallic acid equivalent/g, and total flavonoids were 23.1 ± 0.79 mg rutin equivalent/g. CDE showed a strong antioxidant activity against ABTS free radicals with IC(50) (the half-inhibitory concentration) of 25.5 ± 0.64 μg/mL. In mushroom tyrosinase inhibitory assay, IC(50) values were 2.1 ± 0.98 and 2.27 ± 0.93 mg/mL for monophenolase and diphenolase, respectively. Evaluated by detecting changes of L* (indicated the darkness of sample), a* (indicated the redness of sample), and b* (indicated the yellowness of sample) values in fresh-cut apple slices model, CDE showed a significant antibrowning effect when compared with ascorbic acid. In addition, it was discovered that CDE in combination with 0.5% ascorbic acid exhibited a synergistic antibrowning effect. Meanwhile, CDE was observed to show a potent antimicrobial effect on all of the tested Gram-positive and Gram-negative bacteria. In conclusion, the results of the present research suggested that pine needles of C. deodara could be used as a natural resource of antibrowning and antimicrobial agents in food preservation.
The present study provides a theoretical basis for the potential application of pine needles of C. deodara to be used as a natural resource of antibrowning and antimicrobial agents in food industry.
本研究旨在探讨雪松松针（Cedrus deodara）水溶性提取物的抗褐变和抗菌活性，雪松松针是一种传统中药，也是松针茶的原料。CDE 的总酚含量为 31.4 ± 0.53 mg 没食子酸当量/g，总黄酮含量为 23.1 ± 0.79 mg 芦丁当量/g。CDE 对 ABTS 自由基具有较强的抗氧化活性，IC50（半抑制浓度）为 25.5 ± 0.64 μg/mL。在蘑菇酪氨酸酶抑制试验中，单酚酶和二酚酶的 IC50 值分别为 2.1 ± 0.98 和 2.27 ± 0.93 mg/mL。通过检测新鲜苹果切片模型中 L*（表示样品的黑暗度）、a*（表示样品的红色度）和 b*（表示样品的黄色度）值的变化，CDE 与抗坏血酸相比表现出显著的抗褐变效果。此外，发现 CDE 与 0.5%抗坏血酸联合使用具有协同抗褐变作用。同时，CDE 对所有测试的革兰氏阳性和革兰氏阴性菌均表现出较强的抗菌作用。综上所述，本研究结果表明，雪松松针可作为食品保鲜中天然抗褐变和抗菌剂的来源。
本研究为将雪松松针作为食品工业中天然抗褐变和抗菌剂的潜在应用提供了理论依据。
