Leven R M, Tablin F
Biomedical Division, Lawrence Berkeley Laboratory, University of California, Davis 95616.
Am J Pathol. 1988 Sep;132(3):417-26.
Wistar Furth (WF) rats were studied and compared with Sprague-Dawley (SD) rats to determine if ultrastructural abnormalities in platelets or megakaryocytes could explain their macrothrombocytopenia. WF rats had one third of the platelet count of healthy rats and two times the platelet volume. Megakaryocyte number was decreased and the size of mature stage three megakaryocytes also was decreased. WF platelets had large membranous inclusions, and otherwise showed normal ultrastructural morphology. The WF megakaryocytes showed abnormal aggregates of the demarcation membrane system. Ruthenium red staining was more intense on WF megakaryocytes and platelets, indicating a possible increase in surface mucopolysaccharides. It is possible that abnormal megakaryocyte membrane structure may lead to WF macrothrombocytopenia.
对Wistar Furth（WF）大鼠进行了研究，并与Sprague-Dawley（SD）大鼠进行比较，以确定血小板或巨核细胞的超微结构异常是否可以解释它们的大血小板减少症。WF大鼠的血小板计数是健康大鼠的三分之一，血小板体积是健康大鼠的两倍。巨核细胞数量减少，成熟三期巨核细胞的大小也减小。WF血小板有大的膜性内含物，但其超微结构形态正常。WF巨核细胞显示分界膜系统异常聚集。WF巨核细胞和血小板上钌红染色更强，表明表面粘多糖可能增加。异常的巨核细胞膜结构可能导致WF大鼠出现大血小板减少症。
