Threatte G A, Adrados C, Ebbe S, Brecher G
Am J Clin Pathol. 1984 Jun;81(6):769-72. doi: 10.1093/ajcp/81.6.769.
The time course of artifactual effects due to anticoagulants, specimen temperature, and interval between venipuncture and analysis on platelet volume measurements was evaluated. Split specimens were analyzed using hydrodynamic focusing, and platelet distributions were computed using a least-squares fit to a log-normal distribution. Significant artifacts resulted from exposure to EDTA, cooling to room temperature, and delay in exposure to anticoagulant. The artifactual effect of EDTA is extreme and time dependent. Collection of blood in Buffered Citrate, Acid Citrate Dextrose, or Pyridoxal-5'-phosphate supplemented Citrate yielded stable and equivalent results with rapid anticoagulation and incubation at 37 degrees C for up to six hours.
评估了抗凝剂、样本温度以及静脉穿刺与分析之间的间隔时间对血小板体积测量的人为影响的时间进程。使用流体动力学聚焦对分离的样本进行分析，并使用对数正态分布的最小二乘法拟合计算血小板分布。暴露于乙二胺四乙酸（EDTA）、冷却至室温以及延迟暴露于抗凝剂会导致显著的人为影响。EDTA的人为影响非常严重且与时间相关。在含有缓冲柠檬酸盐、酸性枸橼酸盐葡萄糖或补充了5'-磷酸吡哆醛的柠檬酸盐中采集血液，通过快速抗凝并在37摄氏度下孵育长达6小时，可得到稳定且等效的结果。
