Stickland N C, White R N, Mescall P E, Crook A R, Thorpe J E
Department of Anatomy, Royal Veterinary College, London, United Kingdom.
Anat Embryol (Berl). 1988;178(3):253-7. doi: 10.1007/BF00318228.
From fertilisation to hatching one group of salmon embryos was reared at ambient temperatures (fluctuating around 1.6 degrees C) and another at 10 degrees C. At Gorodilov stages 28, 30 and 33 transverse sections of whole embryos were obtained for light and electron microscopy. Total cross-sectional areas, fibre numbers, fibre diameters and myofibrillar areas of the white muscle of m. lateralis were measured. At hatching (stage 33, which occurred much earlier at the higher temperature), the higher temperature embryos had significantly larger (P less than 0.01) but fewer (P less than 0.05) muscle fibres. These larger fibres contained significantly more myofibrillar material (P less than 0.05) than the smaller fibres of the lower temperature embryos. Lesser differences were found at pre-hatching stages. Higher temperatures caused myofibre hypertrophy to increase at a greater rate than hyperplasia. Hence, the cellularity of the tissue produced under the different temperature regimes was quite different.
从受精到孵化，一组鲑鱼胚胎在环境温度下（约1.6摄氏度波动）饲养，另一组在10摄氏度下饲养。在戈罗季洛夫阶段28、30和33，获取整个胚胎的横切片用于光学显微镜和电子显微镜观察。测量了侧肌白色肌肉的总横截面积、纤维数量、纤维直径和肌原纤维面积。在孵化时（阶段33，在较高温度下出现得更早），较高温度下的胚胎肌肉纤维明显更大（P<0.01）但数量更少（P<0.05）。这些较大的纤维比较低温度下胚胎的较小纤维含有明显更多的肌原纤维物质（P<0.05）。在孵化前阶段发现的差异较小。较高温度导致肌纤维肥大的增加速度超过增生。因此，在不同温度条件下产生的组织细胞构成有很大差异。
