Fujinaga M, Mazze R I, Baden J M, Fantel A G, Shepard T H
Department of Anesthesia, Stanford University School of Medicine, California 94305.
Anesthesiology. 1988 Sep;69(3):401-4.
The teratogenic effects of nitrous oxide (N2O) on postimplantation rat embryos were studied using a whole embryo culture system to separate the direct effects of N2O from those that are maternally mediated. A total of 100, 10-day-old rat embryos were cultured in either a control atmosphere (75% N2, 20% O2, and 5% CO2), or a N2O atmosphere (75% N2O, 20% O2, and 5% CO2). After 22 h of culture embryos were examined microscopically, and protein and DNA contents were determined. DNA content was significantly lower in the embryos exposed to N2O compared with the controls. Additionally, three malformed embryos and four embryos with left-sided tails were observed in the N2O group, whereas no abnormalities were observed in the control group. There were no differences in crown-rump length, somite numbers, limb bud index, and protein content between the two groups of embryos. The positive findings in this study indicate that whole embryo culture is useful for studying the mechanisms of N2O teratogenicity.
利用全胚胎培养系统研究了一氧化二氮（N2O）对植入后大鼠胚胎的致畸作用，以区分N2O的直接作用和母体介导的作用。总共100个10日龄大鼠胚胎分别在对照气体环境（75% N2、20% O2和5% CO2）或N2O气体环境（75% N2O、20% O2和5% CO2）中培养。培养22小时后，对胚胎进行显微镜检查，并测定蛋白质和DNA含量。与对照组相比，暴露于N2O的胚胎中DNA含量显著降低。此外，在N2O组中观察到3个畸形胚胎和4个左侧有尾巴的胚胎，而对照组未观察到异常。两组胚胎在顶臀长度、体节数、肢芽指数和蛋白质含量方面没有差异。本研究的阳性结果表明，全胚胎培养对于研究N2O致畸机制是有用的。
