Ahmadi Mehdi, Niazi Forud, Jaafarzadeh Neematollah, Ghafari Shokouh, Jorfi Sahand
Environmental Technologies Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
Department of Environmental Health Engineering, School of Health, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
J Environ Health Sci Eng. 2021 Jan 27;19(1):445-456. doi: 10.1007/s40201-021-00617-w. eCollection 2021 Jun.
A biosurfactant producing strain was isolated and the rhamnolipid type biosurfactant was extracted for soil washing of a synthetically and naturally hydrocarbon-contaminated soil. Following the primary screening, was selected and the effect of the carbon and nitrogen source and the salinity on biosurfactant production was studied. Of the best results were observed for glucose as a carbon source, NHCl as a nitrogen source and salinity of 1.4%. The produced biosurfactant was a glycolipid type biosurfactant and reduced the surface tension to 32.5 mN/m with a critical micelle concentration (CMC) of 50 mg/L and production yield of 90 mg/L. Using produced biosurfactant, a pyrene desorption rate of 82% was observed in selected conditions for initial pyrene concentration of 200 mg/L.
分离出一株生物表面活性剂产生菌，并提取了鼠李糖脂型生物表面活性剂，用于对合成污染和天然污染的烃类土壤进行土壤淋洗。经过初步筛选，选出了该菌株，并研究了碳源、氮源和盐度对生物表面活性剂产量的影响。结果表明，以葡萄糖为碳源、氯化铵为氮源、盐度为1.4%时效果最佳。所产生的生物表面活性剂为糖脂型生物表面活性剂，可将表面张力降低至32.5 mN/m，临界胶束浓度（CMC）为50 mg/L，产量为90 mg/L。在初始芘浓度为200 mg/L的选定条件下，使用所产生的生物表面活性剂，芘的解吸率为82%。
