商业船舶压载水中[具体物质]的分子检测：沿波斯湾的初步研究
Molecular detection of and in ballast water of commercial ships: a primary study along the Persian Gulf.
作者信息
Soleimani Farshid, Taherkhani Reza, Dobaradaran Sina, Spitz Jörg, Saeedi Reza
机构信息
Systems Environmental Health and Energy Research Center, The Persian Gulf Biomedical Sciences Research Institute, Bushehr University of Medical Sciences, Boostan 19 Alley, Imam Khomeini Street, Bushehr, Iran.
The Persian Gulf Tropical Medicine Research Center, The Persian Gulf Biomedical Sciences Research Institute, Bushehr University of Medical Sciences, Bushehr, Iran.
出版信息
J Environ Health Sci Eng. 2021 Feb 3;19(1):457-463. doi: 10.1007/s40201-021-00618-9. eCollection 2021 Jun.
PURPOSE
Ballast water is one of the most important ways for the transfer of aquatic organisms such as () and . The aim of this study was to investigate gene of and the gene of bacteria by PCR technique in the ballast water of commercial ships entering Bushehr port along the Persian Gulf.
METHODS
In this study, 34 samples of ballast water entered Bushehr port were studied by using culture and PCR methods to determine gene of and gene of . Genomic DNA of bacterial strains was extracted and PCR was performed by using specific primers of and .
RESULTS
The specific gene of was detected in 4 ballast water samples and the positive samples were analyzed by antisera methods for E. coli O157:H7. Results of antisera showed that there were 3 positive samples of O157:H7 serotype. The results of the PCR technique showed that the gene of was negative for all positive culture samples.
CONCLUSIONS
Further studies are highly recommended to monitor other aquatic organisms in ballast water to protect the marine environment.
目的
压载水是诸如（）和（）等水生生物转移的最重要途径之一。本研究的目的是通过PCR技术，对沿波斯湾进入布什尔港的商船压载水中的（）基因和（）细菌的（）基因进行调查。
方法
在本研究中，采用培养和PCR方法对34份进入布什尔港的压载水样本进行研究，以确定（）的（）基因和（）的（）基因。提取细菌菌株的基因组DNA，并使用（）和（）的特异性引物进行PCR。
结果
在4份压载水样本中检测到（）的特异性（）基因，对阳性样本采用抗血清方法检测大肠杆菌O157:H7。抗血清结果显示，有3份O157:H7血清型阳性样本。PCR技术结果显示，所有阳性培养样本的（）基因均为阴性。
结论
强烈建议进一步开展研究，以监测压载水中的其他水生生物，保护海洋环境。
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