Coastal Salinity Tolerant Grass Engineering and Technology Research Center, Ludong University, Yantai, Shandong, P.R. China.
CAS Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan City, China.
J Appl Microbiol. 2022 Jan;132(1):483-494. doi: 10.1111/jam.15186. Epub 2021 Jul 13.
Potassium (K) is a key determinant for plant development and productivity. However, more than 90% of K in the soil exists in an insoluble form. K-solubilizing microbes play an important role in the transformation of insoluble K. Thus, the objective of this study was to evaluate K-dissolving ability of Aspergillus aculeatus (F) and growth-promoting properties in perennial ryegrass.
Perennial ryegrass inoculated with A. aculeatus exhibited enhanced soluble K accompanied with higher growth rate and turf quality, compared with the noninoculated regimen. In addition, A. aculeatus also played a primary role in increasing chlorophyll content and photosynthetic capacity of the plant exposed to LK+F (K-feldspar plus A. aculeatus) treatment, compared with the CK (control, no K-feldspar and A. aculeatus), F (only A. aculeatus) and LK (only K-feldspar) groups. Furthermore, the antioxidase activities (CAT and POD) were significantly increased while the oxidative damage (EL and MDA) was dramatically decreased in the LK+F group compared to the LK (K-feldspar) group. Finally, in perennial ryegrass leaves, the genes expression levels of HAK8, HAK12 and HKT18 were obviously elevated in the LK+F group, compared to the CK, F and LK groups.
We concluded that A. aculeatus could solubilize K from bound form and be considered as K-solubilizing biofertilizer through supplementing K in soil.
Aspergillusaculeatus has the potential to be used as a biofertilizer in sustainable agriculture.
钾（K）是植物发育和生产力的关键决定因素。然而，土壤中超过 90%的 K 以不溶形式存在。溶钾微生物在不溶性 K 的转化中起着重要作用。因此，本研究的目的是评估 Aspergillus aculeatus（F）的溶钾能力及其对多年生黑麦草的促生长特性。
与未接种的处理相比，接种 A. aculeatus 的多年生黑麦草表现出可溶性 K 的增加，同时生长速度和草坪质量也得到提高。此外，与 CK（对照，无钾长石和 A. aculeatus）、F（仅 A. aculeatus）和 LK（仅钾长石）组相比，A. aculeatus 还在增加植物叶绿素含量和光合能力方面发挥了主要作用。暴露于 LK+F（钾长石加 A. aculeatus）处理的植物。此外，与 LK（钾长石）组相比，LK+F 组的抗氧化酶活性（CAT 和 POD）显著增加，而氧化损伤（EL 和 MDA）显著降低。最后，在多年生黑麦草叶片中，与 CK、F 和 LK 组相比，LK+F 组的 HAK8、HAK12 和 HKT18 基因表达水平明显升高。
我们得出结论，A. aculeatus 可以从结合态中溶解 K，并通过补充土壤中的 K 作为溶钾生物肥料。
Aspergillus aculeatus 具有作为可持续农业生物肥料的潜力。
