Li Xiaoning, Zhao Chuncheng, Zhang Ting, Wang Guangyang, Amombo Erick, Xie Yan, Fu Jinmin
Coastal Salinity Tolerant Grass Engineering and Technology Research Center, Ludong University, Yantai, China.
CAS Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan City, China.
Front Microbiol. 2021 Feb 19;12:593722. doi: 10.3389/fmicb.2021.593722. eCollection 2021.
Perennial ryegrass () is a cool-season grass whose growth and development are limited by drought and high temperature. has been reported to promote plant growth and counteract the adverse effects of abiotic stresses. The objective of this study was to assess -induced response mechanisms to drought and heat resistance in perennial ryegrass. We evaluated the physiological and biochemical markers of drought and heat stress based on the hormone homeostasis, photosynthesis, antioxidant enzymes activity, lipid peroxidation, and genes expression level. We found out that under drought and heat stress, -inoculated leaves exhibited higher abscisic acid (ABA) and lower salicylic acid (SA) contents than non-inoculated regimes. In addition, under drought and heat stress, the fungus enhanced the photosynthetic performance, decreased the antioxidase activities, and mitigated membrane lipid peroxidation compared to non-inoculated regime. Furthermore, under drought stress, induced a dramatic upregulation of and and a downregulation of , , and genes. In addition, under heat stress, -inoculated plants exhibited a higher expression level of , , and while a lower expression level of and than non-inoculated ones. Our results provide an evidence of the protective role of in perennial ryegrass response to drought and heat stresses.
多年生黑麦草（）是一种冷季型草，其生长和发育受到干旱和高温的限制。据报道， 可促进植物生长并抵消非生物胁迫的不利影响。本研究的目的是评估 在多年生黑麦草中诱导的抗旱和耐热反应机制。我们基于激素稳态、光合作用、抗氧化酶活性、脂质过氧化和基因表达水平评估了干旱和热胁迫的生理和生化指标。我们发现，在干旱和热胁迫下，接种 的叶片比未接种的对照表现出更高的脱落酸（ABA）含量和更低的水杨酸（SA）含量。此外，在干旱和热胁迫下，与未接种的对照相比，该真菌增强了光合性能，降低了抗氧化酶活性，并减轻了膜脂过氧化。此外，在干旱胁迫下， 诱导了 和 的显著上调以及 、 和 基因的下调。此外，在热胁迫下，接种 的植物比未接种 的植物表现出更高的 、 和 表达水平，而 和 的表达水平更低。我们的结果提供了 在多年生黑麦草对干旱和热胁迫反应中的保护作用的证据。
