Scholtissek C, Müller K
Institut für Virologie, Justus-Liebig-University Giessen, Federal Republic of Germany.
Arch Virol. 1988;101(1-2):119-23. doi: 10.1007/BF01314656.
The infectivity of influenza A viruses like fowl plague virus (FPV) with a cleaved hemagglutinin (HA) is highly sensitive to treatment at pH 5, while strains like PR 8 or virus N with a noncleaved HA survive under this condition. After double infection of chick embryo cells with FPV and PR 8 or virus N, the yield of virus with the HA gene of FPV is greatly reduced. However, it can now survive treatment at pH 5, and the surviving FPV particles form plaques only in the presence of trypsin, indicating that they were coated by the HA of PR 8 or virus N, depending on the coinfecting virus. The results are discussed with respect to the build-up and maintenance of a large reservoir of nonpathogenic influenza A viruses with noncleavable HA in water fowl.
像禽瘟病毒（FPV）这种具有裂解血凝素（HA）的甲型流感病毒的感染性对pH 5处理高度敏感，而像PR 8或具有未裂解HA的病毒N等毒株在这种条件下能够存活。在用FPV和PR 8或病毒N双重感染鸡胚细胞后，具有FPV HA基因的病毒产量大幅降低。然而，它现在能够在pH 5处理下存活，并且存活的FPV颗粒仅在胰蛋白酶存在的情况下形成噬斑，这表明它们被PR 8或病毒N的HA所包裹，具体取决于共感染的病毒。针对水禽中具有不可裂解HA的大量非致病性甲型流感病毒库的形成和维持，对这些结果进行了讨论。
