Scholtissek C
Arch Virol. 1985;85(1-2):1-11. doi: 10.1007/BF01317001.
We have measured the infectivity of influenza A virus strains grown either in embryonated eggs or in chick embryo cells in culture after treatment at low pH. At pH values at which hemolysis occurs there was an irreversible loss of infectivity. The threshold pH, at which the infectivity was lost, depended on the hemagglutinin subtype of the virus strain. All H5 and H7 strains tested were extremely labile at low pH. In contrast, all H3 strains were relatively stable, independent of the species from which the viruses were isolated. With several H1 viruses the hemagglutination (HA) activity was irreversibly lost at intermediate pH values causing inactivation of infectivity. Strains with noncleaved hemagglutinins were much more stable. These observations might explain why duck influenza viruses can easily survive in lake water and wet faeces, and multiply in the intestinal tract, where trypsin is present. There are also significant differences in heat stability exhibited by influenza A strains. In contrast to pH stability this is not a specific trait of the hemagglutinin, since it can be influenced by reassortment. There is no correlation between the stability of infectivity at low pH and heat.
我们已经测量了在低pH处理后，在鸡胚或培养的鸡胚细胞中生长的甲型流感病毒株的感染性。在发生溶血的pH值下，感染性出现不可逆的丧失。感染性丧失的阈值pH取决于病毒株的血凝素亚型。所有测试的H5和H7毒株在低pH下都极其不稳定。相比之下，所有H3毒株相对稳定，与分离病毒的物种无关。对于几种H1病毒，血凝（HA）活性在中等pH值下不可逆地丧失，导致感染性失活。具有未裂解血凝素的毒株更稳定。这些观察结果可能解释了为什么鸭流感病毒能够轻松地在湖水中和湿粪便中存活，并在存在胰蛋白酶的肠道中繁殖。甲型流感毒株在热稳定性方面也存在显著差异。与pH稳定性不同，这不是血凝素的特异性特征，因为它可受重配影响。低pH下感染性的稳定性与热稳定性之间没有相关性。
