School of Public Health, North China University of Science and Technology, Tangshan, Hebei, China.
Experiment Animal Center, North China University of Science and Technology, Tangshan, Hebei, China.
Environ Toxicol. 2021 Sep;36(9):1923-1931. doi: 10.1002/tox.23312. Epub 2021 Jun 22.
Lead (Pb) exposure can cause central nervous system (CNS) damage. The process of Pb neurotoxicity is accompanied by the microglia activation. In addition, microglia activation was observed under the intervention of high-fat diets (HFD). This study was designed to investigate the effect of Pb on the cognitive function of mice with HFD, with focus on the microglia activation in brain. Male C57BL/6J mice, 8 weeks of age, were randomly divided into control, HFD, Pb, and HFD + Pb groups. The results showed that HFD following Pb exposure could exacerbate the learning and memory impairment in mice. Pb exposure could promote microglia activation and increase the expression of M1 microglia marker and decrease the expression of M2 microglia marker in the hippocampus of mice with HFD. Our finding suggested that Pb exposure may aggravate CNS damage by promoting M1 polarization and inhibiting M2 polarization of hippocampal microglia in HFD mice.
铅（Pb）暴露可导致中枢神经系统（CNS）损伤。Pb 神经毒性的过程伴随着小胶质细胞的激活。此外，在高脂肪饮食（HFD）的干预下也观察到了小胶质细胞的激活。本研究旨在探讨 Pb 对 HFD 小鼠认知功能的影响，重点研究大脑中小胶质细胞的激活。8 周龄雄性 C57BL/6J 小鼠被随机分为对照组、HFD 组、Pb 组和 HFD+Pb 组。结果表明，Pb 暴露后 HFD 可加重小鼠的学习和记忆障碍。Pb 暴露可促进小胶质细胞的激活，并增加 HFD 小鼠海马中 M1 小胶质细胞标志物的表达，降低 M2 小胶质细胞标志物的表达。我们的发现表明，Pb 暴露可能通过促进 HFD 小鼠海马中小胶质细胞的 M1 极化和抑制 M2 极化来加重 CNS 损伤。
