Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan, 430070, Hubei, China.
Guangxi Provincial Key Laboratory of Buffalo Genetics, Breeding and Reproduction Technology, Buffalo Research Institute, Chinese Academy of Agricultural Sciences, Nanning, China.
Trop Anim Health Prod. 2021 Jun 22;53(3):366. doi: 10.1007/s11250-021-02713-3.
Fatty acid synthase (FASN) is a multifunctional protein that catalyzes the synthesis of long-chain saturated fatty acid. In this study, we identified the single nucleotide polymorphisms (SNPs), and their association with milk traits in Mediterranean buffalo, and the expression of FASN gene in different tissues was measured. Nine SNPs (g.-1640G > A, g.-1099C > T, g.1095C > A, g.3221G > A, g.4762G > A, g.5299G > A, g.7164G > A, g.7272 T > C, and g.8927 T > C) were identified by DNA pooled sequencing and then genotyped. Seven identified SNPs except g.3221G > A and g.8927 T > C were found significantly associated with both fat and protein percentage, and also the g.7164G > A and g.8927 T > C had significant association with peak milk yield and protein percentage, respectively. One haplotype block was successfully constructed by linkage disequilibrium (LD) analysis and it showed a significant association with both fat percentage and protein percentage. Expression of FASN gene was found in almost all the buffalo tissues including mammary gland, heart, liver, spleen, lung, kidney, uterus, and ovary, and to be highest in lung and mammary gland. Our findings suggest that polymorphisms in the buffalo FASN gene are associated with milk production traits and can be used as a candidate gene for milk traits and marker-assisted selection in buffalo breeding program.
脂肪酸合酶（FASN）是一种多功能蛋白，可催化长链饱和脂肪酸的合成。本研究鉴定了单核苷酸多态性（SNP）及其与地中海水牛乳性状的关联，并测量了 FASN 基因在不同组织中的表达。通过 DNA 池测序鉴定了 9 个 SNP（g.-1640G > A、g.-1099C > T、g.1095C > A、g.3221G > A、g.4762G > A、g.5299G > A、g.7164G > A、g.7272 T > C 和 g.8927 T > C），然后进行了基因分型。除 g.3221G > A 和 g.8927 T > C 外，其余 7 个鉴定 SNP 均与脂肪和蛋白质百分比均显著相关，g.7164G > A 和 g.8927 T > C 与产奶高峰期和蛋白质百分比显著相关。通过连锁不平衡（LD）分析成功构建了一个单倍型块，该单倍型块与脂肪百分比和蛋白质百分比均显著相关。FASN 基因的表达几乎存在于所有水牛组织中，包括乳腺、心脏、肝脏、脾脏、肺、肾脏、子宫和卵巢，在肺和乳腺中表达最高。我们的研究结果表明，水牛 FASN 基因的多态性与产奶性状相关，可作为水牛产奶性状和标记辅助选择的候选基因。
