Deng Tingxian, Pang Chunying, Ma Xiaoya, Lu Xingrong, Duan Anqin, Zhu Peng, Liang Xianwei
Guangxi Provincial Key Laboratory of Buffalo Genetics, Breeding and Reproduction Technology, Buffalo Research Institute, Chinese Academy of Agricultural Sciences, Nanning 530001, China.
Guangxi Provincial Key Laboratory of Buffalo Genetics, Breeding and Reproduction Technology, Buffalo Research Institute, Chinese Academy of Agricultural Sciences, Nanning 530001, China.
Mol Cell Probes. 2016 Oct;30(5):294-299. doi: 10.1016/j.mcp.2016.09.003. Epub 2016 Sep 27.
Insulin-induced genes (INSIGs), including INSIG1 and INSIG2, are important mediators that play a pivotal role in the lipid metabolism and could cause the retention of the SCAP/SREBP complex. Therefore, the objective of this study is to detect the single nucleotide polymorphisms (SNPs) of buffalo INSIG2 gene and evaluate their associations with milk production traits in Chinese buffaloes. A total of four SNPs (g.621272A > G, g.621364A > C, g.632543G > A, and g.632684C > T) were identified using DNA pooled sequencing, and the SNP genotyping for the identified SNPs was performed by using Matrix-assisted laser desorption/ionization time of flight mass spectrometry method from 264 individuals. The results showed that four SNPs were significantly associated with 305-day milk yield or protein percentage in Murrah and crossbred breeds (P < 0.05), but they had no significant effect on milk production traits in Nili-Ravi buffaloes (P > 0.05). Linkage disequilibrium (LD) analysis revealed that one haplotype block was successfully constructed, of which the diplotype H1H1 showed significant association with 305-day milk yield in Murrah buffaloes (P < 0.05). Our findings provide evidence that polymorphisms in buffalo INSIG2 gene are associated with milk production traits, and could be used as a candidate gene for marker-assisted selection in buffalo breeding program.
胰岛素诱导基因（INSIGs），包括INSIG1和INSIG2，是在脂质代谢中起关键作用的重要介质，可导致SCAP/SREBP复合物的滞留。因此，本研究的目的是检测水牛INSIG2基因的单核苷酸多态性（SNPs），并评估它们与中国水牛产奶性状的关联。使用DNA混合测序共鉴定出4个SNP（g.621272A>G、g.621364A>C、g.632543G>A和g.632684C>T），并采用基质辅助激光解吸/电离飞行时间质谱法对264个个体进行了已鉴定SNP的基因分型。结果表明，4个SNP与摩拉水牛和杂交品种的305天产奶量或蛋白质百分比显著相关（P<0.05），但对尼里-拉菲水牛的产奶性状无显著影响（P>0.05）。连锁不平衡（LD）分析表明成功构建了一个单倍型块，其中双倍型H1H1与摩拉水牛的305天产奶量显著相关（P<0.05）。我们的研究结果提供了证据，表明水牛INSIG2基因的多态性与产奶性状相关，可作为水牛育种计划中标记辅助选择的候选基因。
