Department of Cell and Molecular Biology and Microbiology, Faculty of Biological Science and Technology, University of Isfahan, 81746-73441, Isfahan, Iran.
Department of Molecular Genetics, Faculty of Biological Sciences, Tarbiat Modares University, 14115-111, Tehran, Iran.
Sci Rep. 2021 Jun 22;11(1):13064. doi: 10.1038/s41598-021-92603-3.
The association of PRM1/2 with male azoospermia is well-documented, but the relationship between TXNDC2 deficiency and the azoospermia phenotype, sperm retrieval, and pathology has not been elucidated. Here we identified the association of TXNDC2 and protamines in evaluating testis pathology and sperm retrieval. An extensive microarray meta-analysis of men with idiopathic azoospermia was performed, and after undergoing several steps of data quality controls, the data passing QC were pooled and batch effect corrected. As redox imbalance has been shown to have a variable relationship with fertility, our relative expression studies began with candidate protamination and thioredoxin genes. We constructed a logistic regression model of TXNDC2 with PRM1 and PRM2 genes, and collective ROC analysis indicated a sensitivity of 96.8% and specificity of 95.5% with a ROC value of 0.995 (SE = 0.0070, 95% CI 0.982-1.000). These results demonstrate that TXNDC2, PRM1, and PRM2 combined have a robust power to predict sperm retrieval and correlate with severe azoospermia pathology.
PRM1/2 与男性无精子症的关联已有充分的文献记载，但 TXNDC2 缺乏与无精子症表型、精子获取和病理学之间的关系尚未阐明。在这里，我们确定了 TXNDC2 与鱼精蛋白在评估睾丸病理和精子获取中的关联。对特发性无精子症男性进行了广泛的微阵列荟萃分析，经过几个步骤的数据质量控制后，通过 QC 的数据被汇集并进行批处理效应校正。由于氧化还原失衡与生育力的关系具有可变性，我们的候选蛋白和硫氧还蛋白基因的相对表达研究开始了。我们构建了一个包含 TXNDC2 与 PRM1 和 PRM2 基因的逻辑回归模型，集体 ROC 分析表明，TXNDC2 的灵敏度为 96.8%，特异性为 95.5%，ROC 值为 0.995（SE=0.0070，95%CI 0.982-1.000）。这些结果表明，TXNDC2、PRM1 和 PRM2 的组合具有强大的预测精子获取能力，并与严重的无精子症病理相关。
