Zhang Hengzhe, Bao Xiang, Zhang Jian, Hu Qiang, Wei Bingbing
Department of Urology, The Affiliated Wuxi People's Hospital of Nanjing Medical University, Wuxi, China.
Transl Androl Urol. 2021 May;10(5):2113-2121. doi: 10.21037/tau-21-409.
This study aimed to examine the effects of devazepide on the proliferation, migration, and apoptosis of human bladder cancer (BC) 5637 cells, and its mechanism.
A cell counting kit-8 (CCK-8) for cell viability assays, a colony formation assay, and immunofluorescence were applied to detect the effects of devazepide on the proliferation of 5637 cells. Cell cycle assay, cell apoptosis assay and wound healing assay were performed to detect the effects of devazepide on the cell cycle, apoptosis, and migration of 5637 cells. The protein expression of CyclinD1, Bcl-2-associated X protein (Bax), poly ADP-ribose polymerase 1 (PARP1), and Cleaved Caspase-3 in 5637 cells was detected by a western blot assay.
The proliferation of 5637 cells was significantly inhibited (P<0.001) after incubation with 12, 25, and 50 µM devazepide for 48 and 72 h. A treatment of 25 µM devazepide for 48 h induced G1-S cell cycle arrest and apoptosis (P<0.01), and inhibited cell migration (P<0.05). By western blot assay, we found that devazepide can down-regulate CyclinD1 expression, and up-regulate Bax, PARP1, and Cleaved Caspase-3 expression.
Devazepide inhibits the migration and proliferation of human BC 5637 cells by arresting the G1-S cell cycle, and induces cell apoptosis.
本研究旨在探讨地伐西匹对人膀胱癌（BC）5637细胞增殖、迁移和凋亡的影响及其机制。
应用细胞计数试剂盒-8（CCK-8）进行细胞活力检测、集落形成试验和免疫荧光法检测地伐西匹对5637细胞增殖的影响。进行细胞周期检测、细胞凋亡检测和伤口愈合试验以检测地伐西匹对5637细胞周期、凋亡和迁移的影响。通过蛋白质印迹法检测5637细胞中细胞周期蛋白D1（CyclinD1）、Bcl-2相关X蛋白（Bax）、聚ADP核糖聚合酶1（PARP1）和裂解的半胱天冬酶-3（Cleaved Caspase-3）的蛋白表达。
用12、25和50μM地伐西匹孵育48和72小时后，5637细胞的增殖受到显著抑制（P<0.001）。25μM地伐西匹处理48小时可诱导G1-S期细胞周期阻滞和凋亡（P<0.01），并抑制细胞迁移（P<0.05）。通过蛋白质印迹法，我们发现地伐西匹可下调CyclinD1表达，并上调Bax、PARP1和Cleaved Caspase-3表达。
地伐西匹通过阻滞G1-S期细胞周期抑制人BC 5637细胞的迁移和增殖，并诱导细胞凋亡。
