van den Berg G A, Kingma A W, Visser G H, Muskiet F A
University Hospital Groningen, The Netherlands.
Br J Obstet Gynaecol. 1988 Jul;95(7):669-75. doi: 10.1111/j.1471-0528.1988.tb06528.x.
The metabolism and fate of polyamines during cell proliferation, differentiation, and cell loss were investigated by measuring the concentration of polyamines, their conjugates and some of their metabolites in amniotic fluid of 24 subjects, and in urine of 85 women during pregnancy. The increase of putrescine and spermine in acid-hydrolysed urines during pregnancy appeared to be almost completely due to increases in monoacetylated putrescine and N1,N12 diacetylated spermine, respectively. The latter two were the quantitatively most important polyamines in amniotic fluid. In urine, monoacetylated putrescine showed the highest levels at the end of pregnancy, whereas N1,N12 diacetylated spermine reached the highest values at about 32 weeks gestation. It was impossible to establish whether extracellular monoacetylated putrescine is linked either to cell growth or cell loss. The appearance of N1,N12 diacetylated spermine is probably due to cell loss and dependent on the degree of differentiation during fetal development. The decline and eventual disappearance of urinary N1,N12 diacetylated spermine during the first 2 years after birth may be coherent with maturation of the FAD-dependent polyamine oxidase activity.
通过测量24名受试者羊水以及85名孕妇尿液中多胺、其共轭物和一些代谢产物的浓度，研究了细胞增殖、分化和细胞丢失过程中多胺的代谢及去向。孕期酸水解尿液中腐胺和精胺的增加似乎几乎完全分别归因于单乙酰化腐胺和N1,N12 -二乙酰化精胺的增加。后两者是羊水中数量上最重要的多胺。在尿液中，单乙酰化腐胺在孕期结束时水平最高，而N1,N12 -二乙酰化精胺在妊娠约32周时达到最高值。无法确定细胞外单乙酰化腐胺是否与细胞生长或细胞丢失有关。N1,N12 -二乙酰化精胺的出现可能归因于细胞丢失，并取决于胎儿发育过程中的分化程度。出生后前两年尿液中N1,N12 -二乙酰化精胺的下降及最终消失可能与FAD依赖的多胺氧化酶活性的成熟有关。
