Rootman D S, Hasany S M, Basu P K
Department of Ophthalmology, University of Toronto, Canada.
Br J Ophthalmol. 1988 Jul;72(7):545-9. doi: 10.1136/bjo.72.7.545.
No study has yet been done to investigate the changes in endothelial cell size, perimeter, and density that may result from the warming of corneas in MK (McCarey-Kaufman) medium for specular microscopy. In the present investigation eye bank eyes were stored in MK medium at 4 degrees C and rewarmed daily for six days at 37 degrees C before specular photography of the endothelium was performed. These photographs were compared with wet mount preparations stained with trypan blue and alizarin red made from the same corneas and those stored without rewarming for six days. In addition all corneas were qualitatively analysed with the scanning electron microscope (SEM). The data from serial specular photography were insufficient to allow significant conclusions to be drawn about day to day changes in cell morphology. However, analysis of wet mount preparations revealed that cell density and perimeter varied significantly between those corneas rewarmed daily and those held in cold storage for six days. SEM studies showed an intact cell monolayer with cell loss along the folds of corneal endothelium. We therefore concluded that repeated rewarming at 37 degrees C of corneas stored in MK medium at 4 degrees has a deleterious effect on cell morphology and that folds induced by swelling of corneal tissue result in endothelial cell damage with some loss.
尚未开展任何研究来调查在麦凯里 - 考夫曼（McCarey-Kaufman，MK）培养基中对角膜进行加热以用于角膜内皮显微镜检查时，内皮细胞大小、周长和密度可能发生的变化。在本研究中，眼库角膜在4℃下保存在MK培养基中，并在进行角膜内皮镜面摄影前，每天在37℃下复温6天。将这些照片与用锥虫蓝和茜素红染色的湿片标本进行比较，这些湿片标本取自相同的角膜，以及那些未复温保存6天的角膜。此外，所有角膜均用扫描电子显微镜（SEM）进行定性分析。连续角膜内皮镜面摄影的数据不足以就细胞形态的每日变化得出有意义的结论。然而，对湿片标本的分析表明，每天复温的角膜与冷藏6天的角膜之间，细胞密度和周长存在显著差异。扫描电子显微镜研究显示细胞单层完整，但角膜内皮褶皱处有细胞丢失。因此，我们得出结论，在4℃下保存在MK培养基中的角膜在37℃下反复复温会对细胞形态产生有害影响，并且角膜组织肿胀引起的褶皱会导致内皮细胞损伤并伴有一定程度的细胞丢失。
