Bourne W M
Arch Ophthalmol. 1976 Oct;94(10):1799-1800. doi: 10.1001/archopht.1976.03910040573017.
In a method for the routine examination of the endothelium of donor corneas before transplantation, the donor endothelial cells are examined and photographed as they undergo preservation or culture in liquid media. A specular microscope with a modified objective lens is used (working distance, approximately 9 mm). The donor corneas are kept in a special storage container that allows a direct view of the endothelium from outside the container. The endothelium of any cornea preserved in a liquid medium may thus be examined and photographed at high magnification without opening the storage container.
在一种用于移植前供体角膜内皮常规检查的方法中,当供体内皮细胞在液体培养基中进行保存或培养时,对其进行检查和拍照。使用具有改良物镜的镜面显微镜(工作距离约为9毫米)。供体角膜保存在特殊的储存容器中,该容器可从容器外部直接观察内皮。因此,保存在液体培养基中的任何角膜的内皮都可以在不打开储存容器的情况下进行高倍检查和拍照。