通过 CRISPR/Cas 系统在体内沉默/过表达 lncRNAs。

In Vivo Silencing/Overexpression of lncRNAs by CRISPR/Cas System.

机构信息

Oncogenomics Unit, CRL-ISPRO, Pisa, Italy.

Institute of Clinical Physiology, CNR, Pisa, Italy.

出版信息

Methods Mol Biol. 2021;2348:205-220. doi: 10.1007/978-1-0716-1581-2_14.

DOI:10.1007/978-1-0716-1581-2_14

PMID:34160809

Abstract

Long noncoding RNAs (lncRNAs) are implicated in several biological processes and it has been observed that their expression is altered in several diseases. The generation of animal models where selective silencing or overexpression of lncRNAs can be attained is crucial for their biological characterization, since it offers the opportunity to analyze their function at the tissue specific or organismal level. CRISPR/Cas technology is a newly developed tool that allows to easily manipulate the mouse genome, in turn allowing to discover lncRNAs functions in an in vivo context. Here, we provide an overview of how CRISPR/Cas technology can be used to generate transgenic mouse models in which lncRNAs can be studied.

摘要

长链非编码 RNA（lncRNAs）参与了多个生物学过程，并且已经观察到它们的表达在多种疾病中发生了改变。生成能够选择性沉默或过表达 lncRNAs 的动物模型对于它们的生物学特征分析至关重要，因为它提供了在组织特异性或机体水平上分析它们功能的机会。CRISPR/Cas 技术是一种新开发的工具，可用于轻松操纵小鼠基因组，从而能够在体内环境中发现 lncRNAs 的功能。在这里，我们概述了如何使用 CRISPR/Cas 技术来生成可以研究 lncRNAs 的转基因小鼠模型。