miR-98-5p 通过 IGF2BP1/UHRF2 轴促进食管鳞癌细胞的增殖和抑制凋亡。
IGF2BP1/UHRF2 Axis Mediated by miR-98-5p to Promote the Proliferation of and Inhibit the Apoptosis of Esophageal Squamous Cell Carcinoma.
机构信息
Department of Cell Biology and Genetics, Chongqing Medical University, Chongqing, China.
Department of Anesthesiology, The First Affiliated Hospital of Chongqing Medical University, Chongqing, China.
出版信息
Ann Clin Lab Sci. 2021 May;51(3):329-338.
OBJECTIVE
Esophageal squamous cell carcinoma (ESCC) is a common malignant tumor worldwide. Its five-year survival rate has decreased significantly in recent years. This study was aimed at exploring the roles of the IGF2BP1/UHRF2 axis and miR-98-5p in the progression of esophageal squamous cell carcinoma.
METHODS
The ESCC tissues and paracancerous tissues were collected from the 40 patients with ESCC after surgical resection at the First Affiliated Hospital of Chongqing Medical University (Chongqing, China) from January 2019 to January 2020. The clinicopathological characteristics of these patients were analyzed. Gene expression in all specimens was tested to detect miR-98-5p expression. The function of miR-98-5p on ESCC cell proliferation and apoptosis was performed . The relationship between UHRF2, IGF2BP1, and miR-98-5p was analyzed by IP assay, bioinformatics methods, and Western bolt.
RESULTS
The expression of miR-98-5p decreased in 32/40 (80.0%) of the ESCC patient samples. Kaplan-Meier survival analysis of the TCGA cohort grouped by miR-98-5p levels produced significant differences in overall survival (log rank =0.027). miR-98-5p suppressed ESCC progression. IGF2BP1 and UHRF2 promoted ESCC invasion and proliferation, and they inhibited apoptosis through miR-98-5p mediation.
CONCLUSION
miR-98-5p and the IGF2BP1/UHRF2 axis might have the biological functions of regulating cell proliferation and apoptosis in the progression of ESCC, which might provide potential novel targets, such as miR-98-5p, in the treatment of esophageal squamous cell carcinoma.
目的
食管鳞状细胞癌（ESCC）是一种常见的恶性肿瘤，其五年生存率近年来明显下降。本研究旨在探讨 IGF2BP1/UHRF2 轴和 miR-98-5p 在食管鳞状细胞癌进展中的作用。
方法
收集重庆医科大学第一附属医院 2019 年 1 月至 2020 年 1 月 40 例手术切除的 ESCC 患者的 ESCC 组织和癌旁组织，分析患者的临床病理特征。检测所有标本的基因表达，检测 miR-98-5p 的表达。进行 miR-98-5p 对 ESCC 细胞增殖和凋亡功能的检测。通过 IP 检测、生物信息学方法和 Western blot 分析 UHRF2、IGF2BP1 和 miR-98-5p 之间的关系。
结果
在 40 例 ESCC 患者样本中，miR-98-5p 的表达降低了 32/40（80.0%）。TCGA 队列按 miR-98-5p 水平分组的 Kaplan-Meier 生存分析显示总生存差异有统计学意义（log rank=0.027）。miR-98-5p 抑制 ESCC 进展。IGF2BP1 和 UHRF2 通过 miR-98-5p 介导促进 ESCC 侵袭和增殖，并抑制细胞凋亡。
结论
miR-98-5p 和 IGF2BP1/UHRF2 轴可能具有调节 ESCC 进展中细胞增殖和凋亡的生物学功能，这可能为食管鳞状细胞癌的治疗提供 miR-98-5p 等潜在的新靶点。
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