Faculty of Veterinary Medicine, Tabriz Branch, 201583Islamic Azad University, Tabriz, Iran.
Department of Clinical Sciences, Faculty of Veterinary Medicine, 56947University of Tabriz, Tabriz, Iran.
Hum Exp Toxicol. 2021 Nov;40(11):1852-1866. doi: 10.1177/09603271211026723. Epub 2021 Jun 25.
() has several biological activities and medicinal properties. In this study, we elucidated the impact of the on anxiety-related behaviors, reproductive parameters, antioxidant capacity in male rats subjected to chronic restraint stress (CRS).
35 Wistar rats were divided into five groups: control, CRS-control (received normal saline) and three CRS-treated groups received extract (100, 150, and 200 mg/kg body weight) for 21 consequences days. To induce CRS rats, the rats were immobilized for 21 days and received the extract orally. On the last day of treatment, anxiety-related behaviors were assessed through the sucrose preference test (SPT) as well as elevated plus maze (EPM) tests. Corticosterone, luteinizing hormone (LH), and follicle-stimulating hormone (FSH), testosterone levels were evaluated to determine reproductive capacity. Sperm parameters including the total count, motility, and viability were also analyzed. Weight of body, testis and seminal vesicles was measured as well.
The findings revealed that 100, 150, and 200 mg/kg of extract had anxiolytic effects in CRS rats, as confirmed by the EPM test and SPT. In addition, extract could improve fertile capacity and sperm quality to varying degrees. The level of corticosterone had decreased, whereas the level of LH, FSH and testosterone had increased in CRS-treated rats. Moreover, the reduced level of MDA coincided with an increased rate of antioxidant capacity. Our findings suggest that extract could alleviate stress-induced dysfunctions.
Overall, these observations would infer that extract could improve fertility capacity and behavioral impairment in the stress conditions.
Assumption pathway describing the probability underlying mechanism of CRS-induced anxiety and reproductive toxicity and protective effect of AT.
()具有多种生物活性和药用特性。在这项研究中，我们阐明了 对慢性束缚应激（CRS）雄性大鼠焦虑相关行为、生殖参数和抗氧化能力的影响。
35 只 Wistar 大鼠被分为五组：对照组、CRS-对照组（给予生理盐水）和三组 CRS 处理组，分别给予 提取物（100、150 和 200mg/kg 体重）21 天。为了诱导 CRS 大鼠，大鼠被固定 21 天，并口服给予提取物。在治疗的最后一天，通过蔗糖偏好试验（SPT）和高架十字迷宫（EPM）试验评估焦虑相关行为。评估皮质酮、促黄体生成素（LH）和促卵泡激素（FSH）、睾酮水平以确定生殖能力。还分析了精子参数，包括总计数、活力和活力。测量体重、睾丸和精囊的重量。
结果表明，100、150 和 200mg/kg 的 提取物对 CRS 大鼠具有抗焦虑作用，EPM 试验和 SPT 证实了这一点。此外，提取物可以在不同程度上改善生育能力和精子质量。CRS 处理大鼠的皮质酮水平降低，而 LH、FSH 和睾酮水平升高。此外，抗氧化能力增加的同时 MDA 水平降低。我们的研究结果表明，提取物可以缓解应激引起的功能障碍。
总的来说，这些观察结果表明，提取物可以改善应激条件下的生育能力和行为障碍。
描述 CRS 引起的焦虑和生殖毒性以及 AT 保护作用的潜在机制的假设途径。
