Matallah Ahlem, Guezi Rabie, Bairi Abdelmadjid
Department of Biology, Faculty of natural and life sciences, Kasdi Merbah Ouargla university, Algeria.
Laboratory Saharan Bio-Resources, Department of Biology, Faculty of natural and life sciences. Kasdi Merbah Ouargla university, Algeria.
AIMS Neurosci. 2022 May 25;9(2):264-276. doi: 10.3934/Neuroscience.2022014. eCollection 2022.
Several studies have demonstrated that depression include disruptions not only for mental human disorders but also their healthy living. Rodent-based behavioral tests and models are widely used to understand the mechanisms by which stress triggers anxiety-related behaviors. This present study examined the evidence of a chronic restraint stress (CRS) paradigm in male Wistar rats for the progressive nature of depression alongside with related changes in behavior and functions. The body weight was determined, and the behavior tests, including sucrose preference and the open field test were performed. Theses parameters confirme the presence of anxiety-like and depression-like behaviors beside that we will focus on the response of ACTH and testosterone concentrations in rats. The results obtained during the experiment show that CRS led to decrease the time spent in the field center, a decrease of total distance travelled, in the stressed group compared with the control group. A significant increased of ACTH levels and decreased in testosterone hormone levels in the CRS. According to these results the CRS rodent model has value to validating the development for depression.
多项研究表明，抑郁症不仅会扰乱精神疾病，还会影响健康生活。基于啮齿动物的行为测试和模型被广泛用于理解压力引发焦虑相关行为的机制。本研究考察了雄性Wistar大鼠慢性束缚应激（CRS）范式中抑郁症进展性质的证据，以及行为和功能的相关变化。测定了体重，并进行了包括蔗糖偏好和旷场试验在内的行为测试。这些参数证实了焦虑样和抑郁样行为的存在，此外我们还将关注大鼠促肾上腺皮质激素（ACTH）和睾酮浓度的反应。实验结果表明，与对照组相比，CRS导致应激组在旷场中心停留的时间减少，总移动距离缩短。CRS组促肾上腺皮质激素水平显著升高，睾酮激素水平降低。根据这些结果，CRS啮齿动物模型对于验证抑郁症的发展具有价值。
