Sové Richard J, Milkovich Stephanie, Nikolov Hristo N, Holdsworth David W, Ellis Christopher G, Fraser Graham M
Department of Biomedical Engineering, Johns Hopkins University School of Medicine, Baltimore, MD, United States.
Department of Medical Biophysics, University of Western Ontario, London, ON, Canada.
Front Physiol. 2021 Jun 8;12:654928. doi: 10.3389/fphys.2021.654928. eCollection 2021.
Intravital microscopy has proven to be a powerful tool for studying microvascular physiology. In this study, we propose a gas exchange system compatible with intravital microscopy that can be used to impose gas perturbations to small localized regions in skeletal muscles or other tissues that can be imaged using conventional inverted microscopes. We demonstrated the effectiveness of this system by locally manipulating oxygen concentrations in rat muscle and measuring the resulting vascular responses. A computational model of oxygen transport was used to partially validate the localization of oxygen changes in the tissue, and oxygen saturation of red blood cells flowing through capillaries were measured as a surrogate for local tissue oxygenation. Overall, we have demonstrated that this approach can be used to study dynamic and spatial responses to local oxygen challenges to the microenvironment of skeletal muscle.
活体显微镜已被证明是研究微血管生理学的有力工具。在本研究中，我们提出了一种与活体显微镜兼容的气体交换系统，该系统可用于对骨骼肌或其他可用传统倒置显微镜成像的组织中的小局部区域施加气体扰动。我们通过局部操纵大鼠肌肉中的氧气浓度并测量由此产生的血管反应，证明了该系统的有效性。使用氧运输的计算模型来部分验证组织中氧变化的定位，并测量流经毛细血管的红细胞的氧饱和度作为局部组织氧合的替代指标。总体而言，我们已经证明这种方法可用于研究骨骼肌微环境对局部氧挑战的动态和空间反应。
