用于皮下微血管的厘米级宽视野激光扫描光声显微镜
Centimeter-scale wide-field-of-view laser-scanning photoacoustic microscopy for subcutaneous microvasculature .
作者信息
Liao Tangyun, Liu Yuan, Wu Junwei, Deng Lijun, Deng Yu, Zeng Lvming, Ji Xuanrong
机构信息
State Key Laboratory of Precision Electronics Manufacturing Technology and Equipment, Guangdong University of Technology, Guangzhou 510006, China.
T. Liao and Y. Liu contributed equally to this work.
出版信息
Biomed Opt Express. 2021 Apr 28;12(5):2996-3007. doi: 10.1364/BOE.426366. eCollection 2021 May 1.
Abstract
We developed a simple and compact laser-scanning photoacoustic microscopy (PAM) for imaging large areas of subcutaneous microvasculature in vivo. The reflection-mode PAM not only retains the advantage of high scanning speed for optical scanning, but also offers an imaging field-of-view (FOV) up to 20 × 20 mm, which is the largest FOV available in laser-scanning models so far. The lateral resolution of the PAM system was measured to be 17.5 µm. Image experiments on subcutaneous microvasculature in mouse ears and abdomen demonstrate the system's potential for fast and high-resolution imaging for injuries and diseases of large tissues and organs.
摘要
我们开发了一种简单紧凑的激光扫描光声显微镜（PAM），用于对体内大面积皮下微血管进行成像。反射模式PAM不仅保留了光学扫描高扫描速度的优势，还提供了高达20×20毫米的成像视野（FOV），这是迄今为止激光扫描模型中可用的最大视野。PAM系统的横向分辨率经测量为17.5微米。在小鼠耳朵和腹部进行的皮下微血管成像实验证明了该系统在对大型组织和器官的损伤与疾病进行快速高分辨率成像方面的潜力。
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