University of Alberta, Department of Electrical and Computer Engineering, Edmonton, Alberta, Canada, T6G 2V4.
J Biomed Opt. 2013 Feb;18(2):26001. doi: 10.1117/1.JBO.18.2.026001.
The authors demonstrate in vivo dynamic process imaging using a label-free real-time optical-resolution photoacoustic microscope (OR-PAM). This reflection-mode system takes advantage of a 532-nm fiber laser source with a high pulse repetition rate of up to 600 kHz combined with a fast-scanning mirror system. Microvasculature in SCID mouse ears is imaged at near real-time (0.5 fps) for a 1×1 mm2 field of view (FOV) with micron-scale lateral resolution. We also demonstrate imaging of cardiac-induced microhemodynamics in murine microvasculature at real-time frame-rates (30 fps) over a 250×250 μm2 FOV using real-time C-scan OR-PAM with ability to provide sustained imaging with near real-time feedback for focusing and positioning.
作者使用无标记实时光分辨率光声显微镜（OR-PAM）展示了体内动态过程成像。该反射模式系统利用 532nm 光纤激光源，其脉冲重复率高达 600kHz，并结合快速扫描镜系统。对 SCID 小鼠耳朵中的微血管进行近实时（0.5fps）成像，视野（FOV）为 1×1mm²，具有亚微米级的横向分辨率。我们还展示了使用实时 C 扫描 OR-PAM 以 30fps 的实时帧率在 250×250μm² FOV 范围内对小鼠微血管中的心脏诱导微血流动力学进行成像的能力，该系统能够提供近实时反馈，以便持续成像和聚焦定位。
