使用微型内镜对自由活动的小鼠中的成年神经干细胞进行活体成像。
Live imaging of adult neural stem cells in freely behaving mice using mini-endoscopes.
机构信息
CERVO Brain Research Center, Quebec City, QC G1J 2G3, Canada.
Université Laval, Quebec City, QC G1V 0A6, Canada.
出版信息
STAR Protoc. 2021 Jun 12;2(2):100596. doi: 10.1016/j.xpro.2021.100596. eCollection 2021 Jun 18.
During adulthood, the activation of adult neural stem cells (NSCs) has been mostly studied in post-mortem tissues or in anesthetized animals. This protocol presents an approach that allows for the long-term and minimally invasive investigation of adult NSC activation and physiology in freely behaving animals. By combining specific NSC labeling and mini-endoscopic microscopy, live imaging of NSC division and Ca activity can be performed continuously for 2-3 days and even up to several months. For complete details on the use and execution of this protocol, please refer to Gengatharan et al. (2021).
在成年期，成年神经干细胞 (NSC) 的激活主要在死后组织或麻醉动物中进行研究。本方案提供了一种方法，可以在自由活动的动物中对成年 NSC 的激活和生理学进行长期的、微创的研究。通过结合特定的 NSC 标记和微型内窥镜显微镜，可连续 2-3 天甚至长达数月对 NSC 分裂和 Ca 活性进行实时成像。如需了解本方案的详细使用和执行情况，请参考 Gengatharan 等人 (2021)。
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