小鼠成年神经干细胞分离和培养方案。

Department of Molecular Neuroscience, Graduate School of Medicine, Osaka University, 2-2, Yamadaoka, Suita, Osaka 565-0871, Japan.

WPI Immunology Frontier Research Center, Osaka University, 3-1, Yamadaoka, Suita, Osaka 565-0871, Japan.

STAR Protoc. 2021 May 4;2(2):100522. doi: 10.1016/j.xpro.2021.100522. eCollection 2021 Jun 18.

This protocol entails a simple method for isolation, culturing, and differentiation of adult neural stem cells from the dentate gyrus in the hippocampus and the subventricular zone of adult mice. Cultured adult neural stem cells are an important model to investigate stem cell properties such as proliferation and differentiation and to expand the understanding of plasticity in the adult brain. For complete details on the use and execution of this protocol, please refer to Isaksen et al. (2020).

本方案涉及从成年小鼠海马齿状回和侧脑室下区分离、培养和分化神经干细胞的简单方法。培养的成年神经干细胞是研究干细胞特性（如增殖和分化）以及加深对成年大脑可塑性理解的重要模型。如需详细了解本方案的使用和执行情况，请参考 Isaksen 等人（2020 年）的文献。