Univ Rennes, CNRS, INSERM, IGDR (Institut de Génétique et Développement de Rennes)-UMR6290, ERL U1305, 35000 Rennes, France.
Univ Rennes, CNRS, INSERM, IGDR (Institut de Génétique et Développement de Rennes)-UMR6290, ERL U1305, 35000 Rennes, France.
STAR Protoc. 2022 Sep 16;3(3):101493. doi: 10.1016/j.xpro.2022.101493. Epub 2022 Jun 23.
Drosophila neural stem cells (NSCs) divide asymmetrically to generate siblings of different sizes. This model system has proved helpful in deciphering the contribution of polarity cues and the mitotic spindle in asymmetric cell division. Here, we describe a technique we developed to flatten cultured Drosophila brain explants to accurately image the cytoskeleton in live NCSs. We also describe our approach to efficiently remove centrosomes by laser photo-ablation and to measure daughter cell size after NSC division. For complete details on the use and execution of this protocol, please refer to Thomas et al. (2021).
果蝇神经干细胞（NSCs）不对称分裂产生大小不同的两个子细胞。这一模型系统有助于解析细胞极性和有丝分裂纺锤体在不对称细胞分裂中的作用。在这里，我们描述了一种培养的果蝇脑外植体铺平技术，以准确观察活 NSCs 中的细胞骨架。我们还介绍了一种通过激光光消融有效去除中心体并测量 NSC 分裂后子细胞大小的方法。有关此方案的使用和执行的完整详细信息，请参考 Thomas 等人（2021 年）。
