Malinga Nongcebo Z Z, Shobo Christiana O, Molechan Chantal, Amoako Daniel G, Zishiri Oliver T, Bester Linda A
Biomedical Resource Unit, School of Laboratory Medicine and Medical Sciences, College of Health Sciences, University of KwaZulu-Natal, Durban, South Africa.
Antimicrobial Research Unit, College of Health Sciences, University of KwaZulu-Natal, Durban, South Africa.
Microb Drug Resist. 2022 Mar;28(3):306-316. doi: 10.1089/mdr.2020.0546. Epub 2021 Jun 24.
Bacteria that cause life-threatening illnesses in humans are also capable of contaminating hospital surfaces, thus pose as a potential source of infection. This study aimed to investigate the prevalence, genetic diversity, virulence, and antibiotic resistance profile of in South Africa. In a nonoutbreak setting involving four public hospitals, 777 samples were collected in three different wards from 11 different sites. Phenotypic and genotypic methods were used for isolation and identification. The Kirby-Bauer disk-diffusion method was used to examine antibiotic resistance followed by the combination disk method to characterize extended-spectrum β-lactamases (ESBLs). Antibiotic resistance and virulence genes were screened using PCR and clonality was investigated using enterobacterial repetitive intergenic consensus (ERIC)-PCR. Seventy-five (10%) isolates were recovered. These isolates were obtained from all four hospitals and all three wards involved. However, only six frequently touched surfaces were contaminated. Thirty (40%) isolates were characterized as ESBLs showing high resistance to antibiotics and mostly harboring the group one gene. Virulence genes were highly prevalent among all the isolates. ERIC-PCR showed that the isolates recovered from different sites within the same hospital were genetically similar. The study highlighted that can contaminate various surfaces and this persistence allows for the dissemination of bacteria within the hospital environment. The information from this study can assist hospitals to evaluate and improve current infection prevention and control interventions in place.
可导致人类危及生命疾病的细菌也能够污染医院表面，因此构成潜在的感染源。本研究旨在调查南非[具体细菌名称未给出]的流行情况、遗传多样性、毒力和抗生素耐药性特征。在涉及四家公立医院的非暴发环境中，从11个不同地点的三个不同病房采集了777份样本。采用表型和基因型方法进行分离和鉴定。使用 Kirby-Bauer 纸片扩散法检测抗生素耐药性，随后采用组合纸片法鉴定超广谱β-内酰胺酶（ESBLs）。使用 PCR 筛选抗生素耐药性和毒力基因，并使用肠杆菌重复基因间共识（ERIC）-PCR 研究克隆性。共分离出75株（10%）[具体细菌名称未给出]菌株。这些菌株来自所有四家医院和所有三个涉及的病房。然而，只有六个经常接触的表面被污染。30株（40%）菌株被鉴定为 ESBLs，对抗生素表现出高度耐药性，且大多携带第一组基因。毒力基因在所有分离株中高度流行。ERIC-PCR 显示，从同一医院不同地点分离出的菌株在基因上相似。该研究强调[具体细菌名称未给出]可污染各种表面，这种持续性使得细菌能够在医院环境中传播。本研究所得信息可协助医院评估和改进当前的感染预防与控制措施。
