Department of Otorhinolaryngology & Head and Neck Surgery, Istanbul Faculty of Medicine, Istanbul University, Istanbul, Turkey.
Department of Biochemistry, Faculty of Pharmacy, Istanbul Yeni Yuzyil University, Istanbul, Turkey.
Med Oncol. 2021 Jun 25;38(8):87. doi: 10.1007/s12032-021-01538-2.
Oral squamous cell carcinoma patients are exhausted against the powerful chemotherapies, radiotherapies after the surgery, and their immune system is devastated during the process and antibiotic usage become inescapable. Although prescribing an antibiotic might be fraught for such as drug interaction and undesirable proliferation danger, studies still look for the new ideas such as antibiotic combinations that might be safe to use. The antiproliferative and apoptotic outcomes of levofloxacin with cisplatin combination as well as their single usage were examined with WST-1, Caspase-3/BCA and Annexin V methods on SCC-15 cells and a healthy cell line (MRC-5). 24 h treatment of 50 mM single levofloxacin, 50 mM single cisplatin and 50 mM levofloxacin-cisplatin combination resulted in viability rates of SCC-15 cells as 90%, 67% and 80.8%, respectively. Caspase-3 enzyme activity was enhanced 0.92-fold for single levofloxacin, 13.05-fold for single cisplatin and 9.73-fold for the combination of levofloxacin-cisplatin, the total apoptotic activity of single levofloxacin, single cisplatin and levofloxacin-cisplatin combination were observed as 4.88%, 21.14%, 16.21%, respectively on SCC-15. The apoptotic effect of cisplatin on MRC-5 has been shown to be suppressed when combined with levofloxacin. Considering the cell viability, caspase-3, and apoptotic activity results, it's conclude that the levofloxacin-cisplatin combination was also effective compared to the only cisplatin treatment on OSCC cells. The combination has shown less toxicity for healthy cells than single cisplatin treatment. Therefore, our apoptotic findings suggest that the different dosage combinations are necessary to understand the interaction for the treatment of tongue squamous cell carcinoma.
口腔鳞状细胞癌患者在手术后会经历强大的化疗、放疗，其免疫系统受到严重破坏，抗生素的使用也不可避免。尽管开抗生素可能会存在药物相互作用和不良增殖的风险，但研究仍在寻找新的思路，如抗生素组合，这些组合可能是安全的。我们使用 WST-1、Caspase-3/BCA 和 Annexin V 方法，在 SCC-15 细胞和健康细胞系（MRC-5）中研究了左氧氟沙星与顺铂联合以及单独使用的抗增殖和凋亡作用。24 小时 50mM 左氧氟沙星、50mM 顺铂和 50mM 左氧氟沙星-顺铂联合处理后，SCC-15 细胞的存活率分别为 90%、67%和 80.8%。左氧氟沙星单独使用时 Caspase-3 酶活性增强 0.92 倍，顺铂单独使用时 Caspase-3 酶活性增强 13.05 倍，左氧氟沙星-顺铂联合使用时 Caspase-3 酶活性增强 9.73 倍，单独使用左氧氟沙星、顺铂和左氧氟沙星-顺铂联合处理后，SCC-15 的总凋亡活性分别为 4.88%、21.14%和 16.21%。顺铂与左氧氟沙星联合使用时，对 MRC-5 的凋亡作用受到抑制。考虑到细胞活力、Caspase-3 和凋亡活性的结果，与单独使用顺铂相比，左氧氟沙星-顺铂联合治疗对 OSCC 细胞也同样有效。与单独使用顺铂相比，联合治疗对健康细胞的毒性较小。因此，我们的凋亡研究结果表明，为了理解治疗舌鳞状细胞癌的相互作用，需要采用不同的剂量组合。
