Tuan R S, Lamb B T, Jesinkey C B
Department of Biology, University of Pennsylvania, Philadelphia 19104.
Differentiation. 1988 May;37(3):198-204. doi: 10.1111/j.1432-0436.1988.tb00722.x.
Using a cloned cDNA to the mouse placental calcium-binding protein (MCaBP) [Tuan and Kirwin, preceding paper], mRNA transcripts of the MCaBP have been localized in the mouse placenta by in situ cDNA-RNA hybridization. The procedure involved the use of a nonradioactive, biotinylated cDNA probe. Paraffin sections of the mouse chorioallantoic placenta were processed for in situ hybridization using a procedure modified from that of Lo [8]. The results demonstrated that MCaBP mRNA is localized specifically to the fetal trophoblast of the mouse placenta, and is expressed in a development-dependent manner. The MCaBP cDNA also hybridized strongly in situ to sections of human term placenta, giving rise to signals localized specifically to the syncytiotrophoblastic layer of the chorionic villi.
利用克隆的小鼠胎盘钙结合蛋白（MCaBP）的互补DNA（cDNA）[Tuan和Kirwin，前文]，通过原位cDNA-RNA杂交将MCaBP的信使核糖核酸（mRNA）转录本定位到小鼠胎盘。该程序涉及使用非放射性生物素化cDNA探针。采用一种从Lo[8]的方法改良而来的程序，对小鼠绒毛膜尿囊胎盘的石蜡切片进行原位杂交处理。结果表明，MCaBP mRNA特异性定位在小鼠胎盘的胎儿滋养层，并以发育依赖的方式表达。MCaBP cDNA原位杂交时与人足月胎盘切片也有强烈杂交信号，产生的信号特异性定位在绒毛膜绒毛的合体滋养层。
