Tuan R S, Cavanaugh S T
Biochem J. 1986 Jan 1;233(1):41-9. doi: 10.1042/bj2330041.
Mouse chorioallantoic placenta contains a specific calcium-binding protein (MCaBP). A procedure involving gel filtration and ion-exchange chromatography was developed to purify the MCaBP. The MCaBP activity increased as a function of embryonic gestation and was highly specific for Ca2+. The MCaBP is a monomeric protein of Mr 57000, with pI 4.7. Specific antibodies were prepared against the MCaBP and were used to localize the MCaBP to syncytiotrophoblasts of the chorionic villi of mouse chorioallantoic placenta. These properties suggest that the MCaBP may be involved in transplacental calcium transport.
小鼠绒毛尿囊胎盘含有一种特异性钙结合蛋白（MCaBP）。开发了一种涉及凝胶过滤和离子交换色谱的方法来纯化MCaBP。MCaBP活性随胚胎妊娠而增加，并且对Ca2+具有高度特异性。MCaBP是一种分子量为57000、等电点为4.7的单体蛋白。制备了针对MCaBP的特异性抗体，并用于将MCaBP定位到小鼠绒毛尿囊胎盘绒毛膜绒毛的合体滋养层细胞。这些特性表明MCaBP可能参与胎盘钙转运。
