College of Food Science and Engineering, Ocean University of China, No.5, Yushan Road, Qingdao, Shandong Province 266003, P. R. China.
Institute of Physical Chemistry Rocasolano (IQFR), Spanish National Research Council (CSIC), 28006 Madrid, Spain.
J Agric Food Chem. 2021 Jul 7;69(26):7420-7428. doi: 10.1021/acs.jafc.1c02231. Epub 2021 Jun 25.
Development of efficient peptide-based immunotherapy for shrimp allergy relies on the identification of the dominant T-cell epitopes of its major allergen, tropomyosin. In this study, immunoinformatic tools, T-cell proliferation, cytokine release, IgG/IgE binding, and degranulation assays were used to identify and characterize the T-cell epitopes in Lit v 1 in comparison with previously validated B-cell epitopes. The results showed that of the six in silico predicted T-cell epitopes only one (T2: VQESLLKANIQLVEK, 60-74) promoted T-cell proliferation, the release of IL-2, and upregulated secretion of Th2-associated cytokines in the absence of IgG/IgE binding and degranulation activities. These findings support T2 as a candidate for the development of an efficient peptide-based vaccine for the immunotherapy for shrimp-allergic patients.
开发有效的虾过敏原肽免疫疗法依赖于鉴定其主要过敏原肌球蛋白的优势 T 细胞表位。在这项研究中，免疫信息学工具、T 细胞增殖、细胞因子释放、IgG/IgE 结合和脱颗粒测定用于鉴定和表征 Lit v 1 中的 T 细胞表位，并与以前验证的 B 细胞表位进行比较。结果表明，在六个计算机预测的 T 细胞表位中，只有一个（T2：VQESLLKANIQLVEK，60-74）在没有 IgG/IgE 结合和脱颗粒活性的情况下促进 T 细胞增殖、IL-2 的释放，并上调 Th2 相关细胞因子的分泌。这些发现支持 T2 作为开发有效的基于肽的虾过敏患者免疫疗法疫苗的候选物。
