Department of Food Science and Biotechnology, College of Life Science and Biotechnology, Dongguk University, Goyang, 10326, Republic of Korea.
Probiotics Antimicrob Proteins. 2021 Oct;13(5):1452-1457. doi: 10.1007/s12602-021-09813-4. Epub 2021 Jun 25.
The antibiofilm effect of bacteriocin-like inhibitory substance (BLIS) from Enterococcus faecium DB1 against Clostridium perfringens was investigated in the present study. BLIS of E. faecium DB1 significantly reduced biofilm formation by C. perfringens in a dose-dependent manner for 24 and 48 h. In particular, treatment with BLIS of E. faecium DB1 significantly inhibited biofilm formation by C. perfringens on chicken meat and stainless steel coupon surfaces. Moreover, BLIS of E. faecium DB1 decreased the viability of C. perfringens biofilm and planktonic cells, indicating that the reduction of biofilm formation by C. perfringens might be achieved by killing the bacterial cells. Taken together, the present results suggest that BLIS of E. faecium DB1 can be a promising antibiofilm agent to eradicate C. perfringens.
本研究旨在探讨来自屎肠球菌 DB1 的细菌素类似抑制物质 (BLIS) 对产气荚膜梭菌的抗生物膜作用。BLIS 能显著降低产气荚膜梭菌的生物膜形成，呈时间和剂量依赖性，24 和 48 小时后效果明显。特别是，用屎肠球菌 DB1 的 BLIS 处理能显著抑制鸡肉和不锈钢优惠券表面上的产气荚膜梭菌生物膜形成。此外，BLIS 降低了产气荚膜梭菌生物膜和浮游细胞的活力，表明 BLIS 通过杀死细菌细胞来减少生物膜的形成。综上所述，本研究结果表明，屎肠球菌 DB1 的 BLIS 可能是一种有前途的抗生物膜剂，可以根除产气荚膜梭菌。
