Zoghbi S S, Sostman H D, Duberg A C, Lwebuga-Mukasa J, Carbo P L, Gottschalk A
Department of Diagnostic Radiology, Yale University School of Medicine, New Haven, Connecticut 06510.
Invest Radiol. 1988 Aug;23(8):574-8. doi: 10.1097/00004424-198808000-00006.
Plasma fibronectin, a glycoprotein that is a component of blood thrombi, was evaluated for the in vivo scintigraphic detection of pulmonary emboli in dogs. Fibronectin (canine or human) was labeled with either 131I or with 111In and diethylenetriaminepenta-acetic acid (DTPA) as the bifunctional chelating agent using a modification of the mixed anhydride method. The radiolabeled proteins were administered intravenously 20 to 30 minutes after the embolization of a 99mTc-labeled thrombus. The uptake of radioactivity by the embolus was monitored scintigraphically up to 24 hours. At the end of each experiment, the animal was killed and in vitro tissue counting of radioactivity was performed. Comparative study of the 131I- and 111In-labeled agent is presented with particular reference to their pharmacokinetics. The in vivo uptake of radioactivity by the emboli was limited, indicating that radiolabeled fibronectin is not a good scintigraphic agent for the detection of pulmonary emboli.
血浆纤连蛋白是一种作为血栓成分的糖蛋白，对其用于犬类肺栓塞的体内闪烁显像检测进行了评估。使用混合酸酐法的改进方法，用131I或111In以及二乙烯三胺五乙酸（DTPA）作为双功能螯合剂对纤连蛋白（犬源或人源）进行标记。在99mTc标记的血栓栓塞后20至30分钟静脉注射放射性标记的蛋白质。通过闪烁显像监测栓塞物对放射性的摄取，持续24小时。在每个实验结束时，处死动物并进行放射性的体外组织计数。特别参照其药代动力学对131I和111In标记剂进行了比较研究。栓塞物对放射性的体内摄取有限，表明放射性标记的纤连蛋白不是检测肺栓塞的良好闪烁显像剂。
