da Silva Neves Nilvanei Aparecido, Pinto Andressa Zelenski Lara, Melo Fernando Lucas, Maia Laura Marina Siqueira, da Silva Ferreira Raquel, de Carvalho Michellen Santos, de Campos Júnior Fábio Assis, Nunes Marcio Roberto Teixeira, Ribeiro Bergman Morais, Slhessarenko Renata Dezengrini
Programa de Pós-Graduação em Ciências da Saúde, Faculdade de Medicina, Universidade Federal de Mato Grosso (UFMT), 78060-900 Cuiabá, Mato Grosso, Brasil.
Departamento de Biologia Celular, Instituto de Ciências Biológicas, Universidade de Brasília (UNB), Brasília, Distrito Federal, Brasil.
Virus Res. 2021 Sep;302:198494. doi: 10.1016/j.virusres.2021.198494. Epub 2021 Jun 24.
Anophelinae is a widely dispersed Culicidae subfamily that may carry a unique virome. Here we herein report the set of viruses found in 323 salivary glands of 16 anopheline species sampled at Upper Pantanal, Chapada dos Guimarães National Park and Coxipó river basin, South Central Mato Grosso, Brazil, pooled (n = 11) and subjected to high throughput sequencing. Metagenomics revealed the presence of nine viral sequences belonging to novel viruses from seven viral families: Purunga is a putative novel orbivirus sharing 74% and 65% aa identity, respectively, with the VP1 and VP3 segments of Changuinola serogroup, Jaracatiá flavivirus shares 60% amino-acid (aa) identity with Aedes flavivirus. Coxipó dielmovirus and Chapada dielmovirus shared 51% and 39% aa identity with Merida virus. Coloiado-orthomyxo like virus is 57.1-64.8% identical at aa level to Aedes albonnulatus orthomyxo-like virus. Mujica picorna-like virus shares 49% aa identity with Flen picorna-like virus and Chiquitos virus is 50% similar to Ista virus, both from Picornavirales order. Cerrado partiti-like-virus shares 75-86% aa identity with Atrato partiti-like virus 2. We also found the S and L segments of Anopheles triannulatus orthophasmavirus (92% identity) in Anopheles lutzi from Chapada dos Guimarães. The identification of these putative novel viruses underscore the wide dispersion of viruses in culicid hosts contributing to extensions on mosquito virome descriptions.
按蚊亚科是一种广泛分布的蚊科亚科，可能携带独特的病毒组。在此，我们报告在巴西马托格罗索州中南部的上潘塔纳尔湿地、瓜伊马雷斯国家公园查帕达和科西波河流域采集的16种按蚊的323个唾液腺中发现的病毒组，这些样本被合并（n = 11）并进行高通量测序。宏基因组学揭示了属于7个病毒科的9种新型病毒的病毒序列的存在：Purunga是一种假定的新型环状病毒，分别与Changuinola血清群的VP1和VP3片段具有74%和65%的氨基酸同一性，Jaracatiá黄病毒与伊蚊黄病毒具有60%的氨基酸（aa）同一性。科西波迪尔莫病毒和查帕达迪尔莫病毒与梅里达病毒分别具有51%和39%的氨基酸同一性。科洛亚多正粘病毒样病毒在氨基酸水平上与白纹伊蚊正粘病毒样病毒的同一性为57.1 - 64.8%。穆希卡小RNA病毒样病毒与弗伦小RNA病毒样病毒具有49%的氨基酸同一性，奇基托斯病毒与来自小RNA病毒目（Picornavirales order）的伊斯塔病毒相似性为50%。塞拉多分体病毒样病毒与阿特拉托分体病毒样病毒2具有75 - 86%的氨基酸同一性。我们还在瓜伊马雷斯国家公园查帕达的卢茨按蚊中发现了三带喙库蚊正噬菌体病毒的S和L片段（同一性为92%）。这些假定的新型病毒的鉴定强调了病毒在蚊科宿主中的广泛分布，有助于扩展对蚊虫病毒组的描述。
