Lara Pinto Andressa Zelenski de, Santos de Carvalho Michellen, de Melo Fernando Lucas, Ribeiro Ana Lúcia Maria, Morais Ribeiro Bergmann, Dezengrini Slhessarenko Renata
Programa de Pós-Graduação em Ciências da Saúde, Faculdade de Medicina, Universidade Federal de Mato Grosso, Cuiabá, Mato Grosso, Brazil.
Departamento de Biologia Celular, Instituto de Ciências Biológicas, Universidade de Brasília, Brasília, Distrito Federal, Brazil.
PLoS One. 2017 Nov 8;12(11):e0187429. doi: 10.1371/journal.pone.0187429. eCollection 2017.
Viruses may represent the most diverse microorganisms on Earth. Novel viruses and variants continue to emerge. Mosquitoes are the most dangerous animals to humankind. This study aimed at identifying viral RNA diversity in salivary glands of mosquitoes captured in a sylvatic area of Cerrado at the Chapada dos Guimarães National Park, Mato Grosso, Brazil. In total, 66 Culicinae mosquitoes belonging to 16 species comprised 9 pools, subjected to viral RNA extraction, double-strand cDNA synthesis, random amplification and high-throughput sequencing, revealing the presence of seven insect-specific viruses, six of which represent new species of Rhabdoviridae (Lobeira virus), Chuviridae (Cumbaru and Croada viruses), Totiviridae (Murici virus) and Partitiviridae (Araticum and Angico viruses). In addition, two mosquito pools presented Kaiowa virus sequences that had already been reported in South Pantanal, Brazil. These findings amplify the understanding of viral diversity in wild-type Culicinae. Insect-specific viruses may present a broader diversity than previously imagined and future studies may address their possible role in mosquito vector competence.
病毒可能是地球上种类最多样化的微生物。新病毒和变种不断出现。蚊子是对人类最危险的动物。本研究旨在鉴定在巴西马托格罗索州瓜伊马雷斯国家公园塞拉多的一个野生地区捕获的蚊子唾液腺中的病毒RNA多样性。总共66只属于16个物种的库蚊亚科蚊子组成了9个样本池，进行病毒RNA提取、双链cDNA合成、随机扩增和高通量测序，结果显示存在七种昆虫特异性病毒，其中六种代表弹状病毒科（洛贝拉病毒）、楚病毒科（昆巴鲁病毒和克罗阿达病毒）、全病毒科（穆里西病毒）和分体病毒科（阿拉蒂库病毒和安吉科病毒）的新物种。此外，两个蚊子样本池呈现出已在巴西南部潘塔纳尔湿地报道过的凯奥瓦病毒序列。这些发现加深了对野生型库蚊亚科病毒多样性的理解。昆虫特异性病毒可能具有比以前想象的更广泛的多样性，未来的研究可能会探讨它们在蚊子传播能力中可能发挥的作用。
